Sequences from confirmation amplicon


Confirmation sequences for line 241D12 and hit At4g17780 are shown.
Line ID 241D12
Locus At4g17780 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:9880853
Primers used for PCR 17wx - 8474, show primer details
   
Sequence Name 37-K119356-0022-241-D12C-17wx
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer gene specific primer, 17wx: 5'-CTGAGGAAGTGGTGCTAAATTTTC-3'
Sequence (A. th genome BLAST matches underlined) 
>37-K119356-0022-241-D12C-17wx
CTGCGGAGAGATAAAGAATCTCGTTTTTCCTTGGATAATACTTGAAAAGTCTCTTGTGAT
TATCATAGAAGACAATATTTCCTTGCTTAGAAACCGACACCGGGGTGAACCACCTGTTCC
GCCTTTCCCGGGAAGCAACTCTATTCTCTAAATCTATGGAGTAAGTCTTGGTCCATGTTT
CTACTTCTGTATCCATGCTCCAAATCTCTAGTTTCCATTCAGTCTTAGTATAGGTATTGG
CTATGGCCAGGCGGTCCTCAAGGTTCACTATTTGGAAGGTTTCTTGCGTGACACTCATAT
CAGGAACTGAAATGTTATGTAACTCTTCTTTGTGAAGATCCAAGGCTAGTATTGTCCAAG
CCCTCTCAGTAGGTTTGTGTAACCAGTAGATCGATCCATTCGCACACTCCGATTTTCTTA
CCTCAAAGACCTTATAAGGAGGTAAATCTACATGGATCAGCAATGAGTATGATGGTCAAT
ATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTACGC
A
   
Sequence Name 17-K119355-0022-241-D12C-8409
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>17-K119355-0022-241-D12C-8409
GGTCTTGAGGTAGAAATCGGAGTGTGCGAATGGATCGATCTACTGGTTACACAAACCTAC
TGAGAGGGCTTGGACAATACTAGCCTTGGATCTTCACAAAGAAGAGTTACATAACATTTC
AGTTCCTGATATGAGTGTCACGCAAGAAACCTTCCAAATAGTGAACCTTGAGGACCGCCT
GGCCATAGCCAATACCTATACTAAGACTGAATGGAAACTAGAGATTTGGAGCATGGATAC
AGAAGTAGAAACATGGACCAAGACTTACTCCATAGATTTAGAGAATAGAGTTGCTTCCCG
GGAAAGGCGGAACAGGTGGTTCACCCCGGTGTCGGTTTCTAAGCAAGGAAATATTGTCTT
CTATGATAATCACAAGAGACTTTTCAAGTATTATCCAAGGAAAAACGAGATTCTTTATCT
CTCCGCAGACACTTGTGTTATATCTCCTTTTTTTGAAAATTTAGCACCCNTTCCTCA

Last Updated on 06.12.2018 16:11