Sequences from confirmation amplicon


Confirmation sequences for line 241E09 and hit At3g18215 are shown.
Line ID 241E09
Locus At3g18215 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:6241724
Primers used for PCR 18hw - 8474, show primer details
   
Sequence Name 41-K112689-0022-241-E09C-18hw
GenBank Accession LN504137 [GenBank]
Sequencing primer gene specific primer, 18hw: 5'-AGGAGAAGTAGAAAGCCCGTAATC-3'
Sequence (A. th genome BLAST matches underlined) 
>41-K112689-0022-241-E09C-18hw
GTACTCGCAGTGCTCTCTTTTCCCTCTTGAGACAGGCACTGTGACTAGGAAACTTACGTG
AGCGTAATACCTGATTGACTGGATGTTGCAGAGAAAGGCCATGAGGAAACAGATCAGGAT
TGCAAAGTTCTTGAATGACGCTAAGCGAGGGCTTTTGCTCCCGTATATGAGATTTGTTGC
CGTGGATTTTGAAGATGACGAGTTGCTTACAAAGACGCCGATGATGGAACATAAAGTGAT
TGCTGTTGTGGCTAAAAGGGTTGATGCCATTATGTTGTTCCGGATTGTCTGAACAGCCAA
GGTACCATTTTTCAGTGGTTCCTGGAAATAACAATAGATAAACTTATATCATCTACTGCA
AAAGAACCAATACTGAGAGCCAAGACAAACATAAAAAGAAAGAAAGAGTAAATGGCTTCA
TGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAA
GAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCC
   
Sequence Name 09-K112688-0022-241-E09C-8409
GenBank Accession LN490252 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>09-K112688-0022-241-E09C-8409
ATTACTCTTTCTTTCTTTTATGTTTGTCTTGGCTCTCAGTATTGGTTCTTTTGCAGTAGA
TGATATAAGTTTATCTATTGTTATTTCCAGGAACCACTGAAAAATGGTACCTTGGCTGTT
CAGACAATCCGGAACAACATAATGGCATCAACCCTTTTAGCCACAACAGCAATCACTTTA
TGTTCCATCATCGGCGTCTTTGTAAGCAACTCGTCATCTTCAAAATCCACGGCAACAAAT
CTCATATACGGGAGCAAAAGCCCTCGCTTAGCGTCATTCAAGAACTTTGCAATCCTGATC
TGTTTCCTCATGGCCTTTCTCTGCAACATCCAGTCAATCAGGTATTACGCTCACGTAAGT
TTCCTAGTCACAGTGCCTGTCTCAAGAGGGAAAAGAGAGCACTGCGAGTACGTGTCTAGA
AACCTGAACCGAGCTAGCTACTTCTGGTCTCTCGGATTACGGGC

Last Updated on 06.12.2018 16:11