Sequences from confirmation amplicon


Confirmation sequences for line 241F03 and hit At4g24960 are shown.
Line ID 241F03
Locus At4g24960 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:12828715
Primers used for PCR DE42 - 8474, show primer details
   
Sequence Name 75-K105064-0022-241-F03C-DE42
GenBank Accession LN513243 [GenBank]
Sequencing primer gene specific primer, DE42: 5'-TTGATTTCCTCTGTTTCGATTTCT-3'
Sequence (A. th genome BLAST matches underlined) 
>75-K105064-0022-241-F03C-DE42
ATTGTGGTATGTATTGCAGGCCTATCGTGATGTTACTTTATCCATTGTAAGTTTCTCTCT
TGTTAACCCTTTAACAAAAGAAAAAGTTTCTTTCTGGTTCGATCTCTCTACTTTTTGTTG
AAGATTAAAAACAAAAATCTGTGAATATTTACTAATTATGTTTTTGATTACAGGTACGCA
TCGGTGATAGCAATGGAGAGCACAACAAAAGTGGACGATGAACAGTGGCTTGCGTATTGG
ATAATATATTCGTTCCTCTCACTCACGGAACTGATTCTGCAATCGCTTATTGAGTGGATT
CCGATTTGGTATACGGTGAAACTTGTGTTCGTGGCTTGGCTAGTTCTTCCTCAGTTTCAA
GGCGCAGCTTTTATCTACAACCGTGTTGTTAGAGAACAGTTCAAGAAACACGGCGTCCTC
CGCTCCACCCACTCCAAGCCCACCAAGCCCAACATCCTCCACTCCATTTTCCCCCACCGG
GTAAATTTTTTATCTTTTTGGTTTTGAATATTCAATTGTAAATGGCTTCATGTCCGGGAA
ATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTAC
CAATTTTTTTTCAATTCAAAAATGTAAATGTCCG
   
Sequence Name 59-K105065-0022-241-F03C-8409
GenBank Accession LN509996 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>59-K105065-0022-241-F03C-8409
GCATTTACAATTGAATATTCAAAACCAAAAAGATAAAAAATTTACCCGGTGGGGGAAAAT
GGAGTGGAGGATGTTGGGCTTGGTGGGCTTGGAGTGGGTGGAGCGGAGGACGCCGTGTTT
CTTGAACTGTTCTCTAACAACACGGTTGTAGATAAAAGCTGCGCCTTGAAACTGAGGAAG
AACTAGCCAAGCCACGAACACAAGTTTCACCGTATACCAAATCGGAATCCACTCAATAAG
CGATTGCAGAATCAGTTCCGTGAGTGAGAGGAACGAATATATTATCCAATACGCAAGCCA
CTGTTCATCGTCCACTTTTGTTGTGCTCTCCATTGCTATCACCGATGCGTACCTGTAATC
AAAAACATAATTAGTAAATATTCACAGATTTTTGTTTTTAATCTTCAACAAAAAGTAGAG
AGATCGAACCAGAAAGAAACTTTTTCTTTTGTTAAAGGGTTTAACAAGAGAGAAACTTAC
AATGGATAAAGTAACATCACGATAGGCCTGCATATACATTACCAACAAATCGATTACGGT
TTGGATTAGAAATCGAAACAAG

Last Updated on 06.12.2018 16:11