Genotyping and primer details


Genotyping and primer details for 241F03 - At4g24960
Line ID 241F03
Locus At4g24960 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:12828715 of line 241F03 Chr4:12828715
   
Insertion allele amplicon 8474 - DE42
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer DE42: 5'-TTGATTTCCTCTGTTTCGATTTCT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:12828163
   
wt amplicon (over InsPos) 02mf - DE42
wt amplicon start Chr4:12828163
wt amplicon end Chr4:12829144
wt amplicon size 982 bp
Locus specific primer DE42: 5'-TTGATTTCCTCTGTTTCGATTTCT-3'
2nd locus specific primer 02mf: 5'-TACACACCTCATCAGTCATCATCA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11