Sequences from confirmation amplicon


Confirmation sequences for line 242E04 and hit At5g06170 are shown.
Line ID 242E04
Locus At5g06170 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:1870158
Primers used for PCR MR80 - 8474, show primer details
   
Sequence Name 19-K017452-022-242-E04C-MR80
GenBank Accession LN494551 [GenBank]
Sequencing primer gene specific primer, MR80: 5'-AACTTGGATTCCGTGGTTGTATAG-3'
Sequence (A. th genome BLAST matches underlined) 
>19-K017452-022-242-E04C-MR80
CATCTCCTGCTGAGTCTCCACCATACACTTCACGACCCATCCAATCGGTATCGTATAAAA
GAAAAGGAAACCATGCGATCCAGTTCAAGGCCGTGACGGCTAGAAGCATCCACATTGGAC
GTTTCATCACCTTGAAAGCTCCAAAGATCTCGCCAAAGAAGGGAGTTTTCTCATTGTCGG
AATCTGCATTAGGCGACCATTGCTTGTCTTCCACGTACCAAAGAGCTATGATTGTGAGGA
CTATGAGGAGAGTGATGGAAATAATGAAACAGCTCTTGAGATTTGCACAATAGATGTCGC
ATGCTTTAGTCACTGTGAAAGGGAAAATCTTGTGGAGGTTAGTGTACGATCCCGCAGCGT
ATCCCAAAACGTTTCCAACGGCCATGAAGAAAGAGAAAATCGCGTTGGCAGTTCGAGTTT
TCTTAGCATCTCCGGCTGCTAAATCACCAAGAAAAGCACGGCAAGGTCCTTGAAGAGTGT
TGTTAGCCACGTCAAGGATCCAGAAACCGACAACGAAGAAACCAACGGCTCTTATCTTGA
CAGCTTCGTCGAGTTTATCTCCCATGGTGTGGCCGAAGTAAATTGTAAATTGTACTCCAT
ATTTTACAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAG
CAATGAGTATGATGGTCAATATGGA
   
Sequence Name 20-K017452-022-242-E04C-8409
GenBank Accession LN495003 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>20-K017452-022-242-E04C-8409
CATGTAGATTTCCCGGAATGAAGCCCTTTACAATTGAATATATCCTGTAAAATATGGAGT
ACAATTTACAATTTACTTCGGCCACACCATGGGAGATAAACTCGACGAAGCTGTCAAGAT
AAGAGCCGTTGGTTTCTTCGTTGTCGGTTTCTGGATCCTTGACGTGGCTAACAACACTCT
TCAAGGACCTTGCCGTGCTTTTCTTGGTGATTTAGCAGCCGGAGATGCTAAGAAAACTCG
AACTGCCAACGCGATTTTCTCTTTCTTCATGGCCGTTGGAAACGTTTTGGGATACGCTGC
GGGATCGTACACTAACCTCCACAAGATTTTCCCTTTCACAGTGACTAAAGCATGCGACAT
CTATTGTGCAAATCTCAAGAGCTGTTTCATTATTTCCATCACTCTCCTCATAGTCCTCAC
AATCATAGCTCTTTGGTACGTGGAAGACAAGCAATGGTCGCCTAATGCAGATTCCGACAA
TGAGAAAACTCCCTTCTTTGGCGAGATCTTTGGAGCTTTCAAGGTGATGAAACGTCCAAT
GTGGATGCTTCTAGCCGTCACGGCCTTGAACTGGATCGCATGGTTTCCTTTTCTTTTATA
CGATACCGATTGGATGGGTCGTGAAGTGTATGGTGGAGACTCAGCAGGAGATGACAAATG

Last Updated on 06.12.2018 16:11