Sequences from confirmation amplicon


Confirmation sequences for line 242E12 and hit At2g14880 are shown.
Line ID 242E12
Locus At2g14880 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:6394805
Primers used for PCR TS02 - 8474, show primer details
   
Sequence Name 15-K025042-022-242-E12C-8409
GenBank Accession LN492803 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>15-K025042-022-242-E12C-8409
AGATTTCCCGGACATGAAGCCATTTACAATTGAATTGCATCAAAACGACATCTTTCCGCA
ACCCATCAACTCCTCTGTCCTCTCTCCGGTTCGCCTCTCACCCGGCGGCTAAACTACGCT
TGGTTCGAGCGGTAACATCTGCGACGGAATCTTCAGAGCCAACCGCAACCAATAAGAGAG
TGCCACGCGGTATCATGAAACCTAGACCAGTTTCACCAGAGATGCAAGACATCGTCGAAC
TTCCTGAAATCGCTCGCACACAAGCTCTTAAGCGTATTTGGGCTTACATCAAAGAACATG
ACCTTCAGGTAACTTAACAACCAAGAATCTCTCTCAGAAACACTCTGGTTTTGGTTTCTC
TTATGGTTTTGTTTCG
   
Sequence Name 16-K025042-022-242-E12C-TS02
GenBank Accession LN493272 [GenBank]
Sequencing primer gene specific primer, TS02: 5'-AGCGAAACAAAACCATAAGAGAAA-3'
Sequence (A. th genome BLAST matches underlined) 
>16-K025042-022-242-E12C-TS02
AACCAGAGTGTTTCTGAGAGAGATTCTTGGTTGTTAAGTTACCTGAAGGTCATGTTCTTT
GATGTAAGCCCAAATACGCTTAAGAGCTTGTGTGCGAGCGATTTCAGGAAGTTCGACGAT
GTCTTGCATCTCTGGTGAAACTGGTCTAGGTTTCATGATACCGCGTGGCACTCTCTTATT
GGTTGCGGTTGGCTCTGAAGATTCCGTCGCAGATGTTACCGCTCGAACCAAGCGTAGTTT
AGCCGCCGGGTGAGAGGCGAACCGGAGAGAGGACAGAGGAGTTGATGGGTTGCGGAAAGA
TGTCGTTTTGATGCAATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCA
GCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAA
TTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGA
CAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAA
ATCTTTATTTCGACGTGTCTACATTCA

Last Updated on 06.12.2018 16:11