Sequences from confirmation amplicon


Confirmation sequences for line 242H07 and hit At1g55840 are shown.
Line ID 242H07
Locus At1g55840 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:20874325
Primers used for PCR MP41 - 8474, show primer details
   
Sequence Name 19-K024603-022-242-H07C-8409
GenBank Accession LN494596 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>19-K024603-022-242-H07C-8409
GTAGATTTCCCGGACATGAAGCCATTTACAATTGAATATATCCTGAATCTTAAATAACTA
ATGCAAAGAGAGAGACTTTGATTCAAATTTAGATCCAGATCACTGAAACCAATATACATG
CCGATCAAATTACACAGGACACGAAAAAAGGGGATATTTTTTTCCCGGTTTTCCCAGAAA
CCTATTTATAGAAAATCGAAAGTATCGATCTTTGGTTCAATAAGCAAAGACTTGGGAGTT
GAATCTTTCAAAGCATCTAAAAAACAAGTGAATTTACTAACCCGATACGACTCTCTGAGT
GAATCATCAACTGTCAATACCAAAAGTAAGTAAAATTAGCTCAAAATTTGAGATTTCGTC
AGCGATCTTCAAACAAAGCAAGCTTAAAAGTTAAAACATTGAAAACCCAAATAAAGACAC
ACCATCTTCCATTAAAGCACGCAATTGCTTCACTGCTTCTTCATTAGTGATGCTCATTTT
CTCACTCTAGAGATCTCAGCTCAGATTTGACGAATACA
   
Sequence Name 20-K024603-022-242-H07C-MP41
GenBank Accession LN495050 [GenBank]
Sequencing primer gene specific primer, MP41: 5'-ACTTTTGCCCACAATTTTGTATTC-3'
Sequence (A. th genome BLAST matches underlined) 
>20-K024603-022-242-H07C-MP41
CAAATCTGAGCTGAGATCTCTAGAGTGAGAAAATGAGCATCACTAATGAAGAAGCAGTGA
AGCAATTGCGTGCTTTAATGGAAGATGGTGTGTCTTTATTTGGGTTTTCAATGTTTTAAC
TTTTAAGCTTGCTTTGTTTGAAGATCGCTGACGAAATCTCAAATTTTGAGCTAATTTTAC
TTACTTTTGGTATTGACAGTTGATGATTCACTCAGAGAGTCGTATCGGGTTAGTAAATTC
ACTTGTTTTTTAGATGCTTTGAAAGATTCAACTCCCAAGTCTTTGCTTATTGAACCAAAG
ATCGATACTTTCGATTTTCTATAAATAGGTTTCTGGGAAAACCGGGAAAAAAATATCCCC
TTTTTTCGTGTCCTGTGTAATTTGATCGGCATGTATATTGGTTTCAGTGATCTGGATCTA
AATTTGAATCAAAGTCTCTCTCTTTGCATTAGTTATTTAAGATTCAGGATATATTCAATT
GTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATAT
GGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAG
CGTTATTATAAAATGAAAGTACATTTTNGATAAAACGACAAATTACGATCCGTCGTATTT
AT

Last Updated on 06.12.2018 16:11