Sequences from confirmation amplicon


Confirmation sequences for line 243A01 and hit At3g23780 are shown.
Line ID 243A01
Locus At3g23780 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:8568011
Primers used for PCR HF31 - 8474, show primer details
   
Sequence Name 33-K032416-022-243-A01C-HF31
GenBank Accession LN500885 [GenBank]
Sequencing primer gene specific primer, HF31: 5'-TACAACGGTAGATCAGGTGAGATG-3'
Sequence (A. th genome BLAST matches underlined) 
>33-K032416-022-243-A01C-HF31
GCCACTTTCACCAGCGACTTGTCCCATGTCAGAGGACAAAGTCAAGTTCAGGAACACTGG
ACCAGTCCACCCGCTCACACGCCAGCCAGTTGCAGACAGGAAGAGATTTGGCGGGATAAA
ATTTGGAGAAATGGAGCGAGACTGCCTAATAGCTCACGGTGCATCAGCTAATCTGCATGA
GCGTCTCTTCACTCTAAGTGACTCTTCTCAGATGCACATCTGCAGAAAATGTAAGACCTA
TGCGAATGTGATCGAGAGGACTCCAAGCAGTGGAAGAAAGATTAGAGGGCCATATTGTAG
AGTCTGCGTATCCTCAGACCATGTGGTTAGGGTCTATGTTCCGTATGGAGCTAAGCTTCT
GTGTCAGGAGCTGTGTCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTA
CATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATT
TTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTG
ATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCT
AATTCGGAAATCTTTATTTCGACGTGTCTACTT
   
Sequence Name 33-K032416-022-243-A01C-8409
GenBank Accession LN500884 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>33-K032416-022-243-A01C-8409
GCATTTACAATTGAATATATCCTGACACAGCTCCTGACACAGAAGCTTAGCTCCATACGG
AACATAGACCCTAACCACATGGTCTGAGGATACGCAGACTCTACAATATGGCCCTCTAAT
CTTTCTTCCACTGCTTGGAGTCCTCTCGATCACATTCGCATAGGTCTTACATTTTCTGCA
GATGTGCATCTGAGAAGAGTCACTTAGAGTGAAGAGACGCTCATGCAGATTAGCTGATGC
ACCGTGAGCTATTAGGCAGTCTCGCTCCATTTCTCCAAATTTTATCCCGCCAAATCTCTT
CCTGTCTGCAACTGGCTGGCGTGTGAGCGGGTGGACTGGTCCAGTGTTCCTGAACTTGAC
TTTGTCCTCTGACATGTGGACAAGTCGCTGGTAGAAAGTTGGGCCCATGAATATCATAGA
ACGCATCATCTCACCTGA
Primers used for PCR FQ27 - 3144, show primer details
   
Sequence Name 46-K033040-022-243-A01C-3144
GenBank Accession LN505777 [GenBank]
Sequencing primer T-DNA primer, 35St: 5'-GTGGATTGATGTGATATCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>46-K033040-022-243-A01C-3144
TCCACTATCCTTCGCAGACCCTTCCTCTATATAAGGAAGTTCATTTCATTTGGAGAGGAC
AGGGTACCCGGGGATCAGATTGTCGTTTCCCGCCTTCGGTTTAAACTATCAGATTGTCCA
CATGTCAGAGGACAAAGTCAAGTTCAGGAACACTGGACCAGTCCACCCGCTCACACGCCA
GCCAGTTGCAGACAGGAAGAGATTTGGCGGGATAAAATTTGGAGAAATGGAGCGAGACTG
CCTAATAGCTCACGGTGCATCAGCTAATCTGCATGAGCGTCTCTTCACTCTAAGTGACTC
TTCTCAGATGCACATCTGCAGAAAATGTAAGACCTATGCGAATGTGATCGAGAGGACTCC
AAGCAGTGGAAGAAAGATTAGAGGGCCATATTGTAGAGTCTGCGTATCCTCAGACCATGT
GGTTAGGGTCTATGTTCCGTATGGAGCTAAGCTTCTGTGTCAGGAGCTGTGTCAGGATAT
ATTCAATTGTAAATGGCTTATTGCCTTAAGACCATGTTATGTGTAGTTTGCTTCAGTCCC
GGTTCTGGTTAGTAGTATAGGTTTTGGTTTGGTTGATTCGGTAAGGGTTATCCGAACCGA
AGAAATCGTAAAACCGAGCCACTGATGACTGAACTAACCCGTAAGTGTTGCTTTTGTGAG
ATTTGACTCTTTAACCGTTAATAATTCTCGGATCTAAAGTAAAGTTTTAGGAAAACTTTC
ATTGTCTTCTTCTTCTTCCGGAGGCATAAACCCCTTGCTCGGAGTCGTT
   
Sequence Name 14-K033040-022-243-A01C-FQ27
GenBank Accession LN492404 [GenBank]
Sequencing primer gene specific primer, FQ27: 5'-ATCGAAGAATGAGAGAAACGACTC-3'
Sequence (A. th genome BLAST matches underlined) 
>14-K033040-022-243-A01C-FQ27
GTAGCCTCGGAAGAGAGAAGACATGAAAGTTTTCCTAAAACTTTACTTTAGATCCGAGAA
TTATTAACGGTTAAAGAGTCAAATCTCACAAAAGCAACACTTACGGGTTAGTTCAGTCAT
CAGTGGCTCGGTTTTACGATTTCTTCGGTTCGGATAACCCTTACCGAATCAACCAAACCA
AAACCTATACTACTAACCAGAACCGGGACTGAAGCAAACTACACATAACATGGTCTTAAG
GCAATAAGCCATTTACAATTGAATATATCCTGACACAGCTCCTGACACAGAAGCTTAGCT
CCATACGGAACATAGACCCTAACCACATGGTCTGAGGATACGCAGACTCTACAATATGGC
CCTCTAATCTTTCTTCCACTGCTTGGAGTCCTCTCGATCACATTCGCATAGGTCTTACAT
TTTCTGCAGATGTGCATCTGAGAAGAGTCACTTAGAGTGAAGAGACGCTCATGCAGATTA
GCTGATGCACCGTGAGCTATTAGGCAGTCTCGCTCCATTTCTCCAAATTTTATCCCGCCA
AATCTCTTCCTGTCTGCAACTGGCTGGCGTGTGAGCGGGTGGACTGGTCCAGTGTTCCTG
AACTTGACTTTGTCCTCTGACATGTGGACAATCTGATAGTTTAAACCGAAGGCGGGAAAC
GACAATCTGATCCCCGGGTACCCTGTCCTCTCCAAATGAAATGAACTTCCTTATATAGAG
GAAGGGTCTTGCGAAGGATAGTGGGATTGTGCGTCATCCCTTACGTCAGTGGAGATATCA
CATCAATCCACTTGCTTTGAAGACGTGGTTGGAACGTCTTCTTTTTCCACGATGCTCCTC
GTGGT
Primers used for PCR 37wp - 8474, show primer details
   
Sequence Name 50-K118546w-0022-243-A01C-37wp
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer gene specific primer, 37wp: 5'-TGATATCATTAAGGGCAGGAACAT-3'
Sequence (A. th genome BLAST matches underlined) 
>50-K118546w-0022-243-A01C-37wp
TTTAACTTGGAGACTTTGGCTACGAATTTCCAAAAGTTGATTGAGCTAATATACTGCACA
AGGCACTAAGCTAGATTTGAGCACCTTACTTGAAACATGTCATAGTGGATCTTTATTTTG
TTTTCTAGGAGTACTAGAAGTGAGCATGAGTTATCTGTCTCTGTAAGACTAAAAAACTAA
GAAGTTCAATGTTCTATGGTTGATTAATTTCTTGTATTGTGCCTGAAAAACGTCTAGAGA
ATACAGAAAAATAGGCTCAAGAGTCATGTACCAGTATATAATTAGTTAGAGGATTGATGC
TGTGAGCCTTGTGATCTATGTATAATTCATTTAACCTTTCTTAGATTATTGTTGATTCTT
GAGTCCTGATTCATTACCAATGGTAAATATTTGTGATTAGGGCCGGCTTTTCAAGATGGG
GAAACGAAAGGGTCTACAACGGTAGATCAGGTGAGATGATGCGTTCTATGATATTCATGG
GCCCAACTTTCTACCAGCGACTTGTCCACATGTCAGAGGACAAAGTCAAGTTCAGGAACA
CTGGACCAGTCCACCCGCTCACACGCCAGCCAGTTGCAGACAGGAAGAGATTTGGCGGGA
TAAAATTTGGAGAAATGGAGCGAGACTGCCTAATAGCTCACGGTGCATCAGCTAATCTGC
ATGAGCGTCTCTTCACTCTAAGTGACTCTTCTCAGATGCACATCTGCAGAANATGTAAGA
CCTATGCGAATGTGATCGAGAGGACTCCAAGCAGTGGAAGAAAGATTAGAGGGCCATATT
GTAGAGTCTGCGTATCCTCAGACCATGTGGTTAGGGTCTATGTTCCGTATGGAGCTAAGC
TTCTGTGTCAGGAGCTGTGTCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAA
TCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAA
   
Sequence Name 30-K118545w-0022-243-A01C-8409
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>30-K118545w-0022-243-A01C-8409
ATTTCATTGAATAATCCTGACACAGCTCCTGACACAGAAGCTTAGCTCCATACGGAACAT
AGACCCTAACCACATGGTCTGAGGATACGCAGACTCTACAATATGGCCCTCTAATCTTTC
TTCCACTGCTTGGAGTCCTCTCGATCACATTCGCATAGGTCTTACATTTTCTGCAGATGT
GCATCTGAGAAGAGTCACTTAGAGTGAAGAGACGCTCATGCAGATTAGCTGATGCACCGT
GAGCTATTAGGCAGTCTCGCTCCATTTCTCCAAATTTTATCCCGCCAAATCTCTTCCTGT
CTGCAACTGGCTGGCGTGTGAGCGGGTGGACTGGTCCAGTGTTCCTGAACTTGACTTTGT
CCTCTGACATGTGGACAAGTCGCTGGTAGAAAGTTGGGCCCATGAATATCATAGAACGCA
TCATCTCACCTGATCTACCGTTGTAGACCCTTTCGTTTCCCCATCTTGAAAAGCCGGCCC
TAATCACAAATATTTACCATTGGTAATGAATCAGGACTCAAGAATCAACAATAATCTAAG
AAAGGTTAAATGAATTATACATAGATCACAAGGCTCACAGCATCAATCCTCTAACTAATT
ATATACTGGTACATGACTCTNGAGCCTATTTTTCTGTATTCTCTAGACGTTTTTCAGGCA
CAATACAAGAAATTAATCAACCATAGAACATTGAACTTCTTAGTTTTTTAGTCTTACAGA
GACAGATAACTCATGCTCACTTCTAGTACTCCTAGAAAACAAAATAAAGATCCACTATGA
CATGTTTCAAGTAAGGTGCTCAAATCTAGCTNAGTGCCTTGTGCAGTATATTAGCTCAAT
CAACTTTTGGAAATTCTGTAGCCAAAGTCTCCAAGTTTAAACATNCTTCACGAGGAAATA
TTATCAATAATATGTTC

Last Updated on 06.12.2018 16:11