Sequences from confirmation amplicon


Confirmation sequences for line 243B02 and hit At2g19170 are shown.
Line ID 243B02
Locus At2g19170 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:8315426
Primers used for PCR CR18 - 8474, show primer details
   
Sequence Name 23-K017723-022-243-B02C-CR18
GenBank Accession LN496356 [GenBank]
Sequencing primer gene specific primer, CR18: 5'-AACATAATTGGGTTCATCTGTTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>23-K017723-022-243-B02C-cr18
CAGCCCATATGAGATAACCGGGTGCAAGAATATCCGGTTTAAGAAGATCAGCATCTTGAA
AGCTGAAATCTTTGGTGTTGGGTCCTCTAGCTGAGAACAATGCCACTTGAGGTGCTGATT
TGTGAAGAACGGGTGCTAAGCCATCTCCGATGCTTCCCTCGGCTTTAAAGCTTTTTACTC
GTCCTGTCCAGTCCCTTGATGTACTGGCGTTGTAGTAATCGATCAAATCCTAAAGATGCA
AAAGACAGCATTTCAGATAGAGAAACTAGGCTAAGAGTAAGTGAAAGCATCAAAACTCTC
ATTACCATTGACTTGGACACATCTGTAATCAGGATTCCTGGAATGGCAGAAGGAACTGGA
TCGAATTTGGTTCCCGGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGA
GTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAA
ATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTA
CGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTA
TTTCGACGTGTCTACATTCACGTCCCAAA
   
Sequence Name 24-K017723-022-243-B02C-8409
GenBank Accession LN496798 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>24-K017723-022-243-B02C-8409
CATGTAGATTTCCCGGACATGAAGCCATTTACCGGGAACCAAATTCGATCCAGTTCCTTC
TGCCATTCCAGGAATCCTGATTACAGATGTGTCCAAGTCAATGGTAATGAGAGTTTTGAT
GCTTTCACTTACTCTTAGCCTAGTTTCTCTATCTGAAATGCTGTCTTTTGCATCTTTAGG
ATTTGATCGATTACTACAACGCCAGTACATCAAGGGACTGGACAGGACGAGTAAAAAGCT
TTAAAGCCGAGGGAAGCATCGGAGATGGCTTAGCACCCGTTCTTCACAAATCAGCACCTC
AAGTGGCATTGTTCTCAGCTAGAGGACCCAACACCAAAGATTTCAGCTTTCAAGATGCTG
ATCTTCTTAAACCGGATATTCTTGCACCCGGTTATCTCATATGGGCTGCTTGGTGTCCAA
ATGGAACAGATGAACCCAATTATG

Last Updated on 06.12.2018 16:11