Sequences from confirmation amplicon


Confirmation sequences for line 243D08 and hit At5g62210 are shown.
Line ID 243D08
Locus At5g62210 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:24986978
Primers used for PCR IN48 - 8474, show primer details
   
Sequence Name 42-K033543-022-243-D08C-IN48
GenBank Accession LN504367 [GenBank]
Sequencing primer gene specific primer, IN48: 5'-TTTCAAGAAGACAACAATGGAGTG-3'
Sequence (A. th genome BLAST matches underlined) 
>42-K033543-022-243-D08C-IN48
TCTGCTTCACTCATCCCATTCCTTCTCTAACCTTCGAGCCACTAATCTCTTCTCTTGATC
TTCATGAAGAAGAAAGCTGCCCTTACACTGTAATTGTTACCACAAGCTGTTTCTCTCCTG
ATTGGTCTAGAGATCAGGTCACTATCGCTTTGGGTGACGCCGACGATAACCAGGTTTGAT
ATACAAAAACTCTTTGGTTGTTTTGTTCTATTTGTGGGTCCAAATATTTGTTATGTTCGT
ATTATTTCAAACCCTGAAGTTATACATATTCCATCTTGTTTGATGGATTCACGTAAGAAG
TTTGTTAAACTCCGGATAAACCAAAGCCATTAGAACCTTAATGAAACTTATTTTGAATAT
AACAGTCTTATTTTGATGGATTTTAGTGACGATGCATGTGTTATGTGTATCTTATTAATA
AATACGTATATCATTGAGTTTTGGTTTTAATTTATTAGGTCCGGGAAATGGCTATATTCA
ATTGATCCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCA
GCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAA
TTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGA
CAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAA
ATCTTTATTTCGACGTGTCTACATT
   
Sequence Name 42-K033543-022-243-D08C-8409
GenBank Accession LN504366 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>42-K033543-022-243-D08C-8409
TTACAATTGAATATATCCTGGATCAATTGAATATAGCCATTTCCCGGACCTAATAAATTA
AAACCAAAACTCAATGATATACGTATTTATTAATAAGATACACATAACACATGCATCGTC
ACTAAAATCCATCAAAATAAGACTGTTATATTCAAAATAAGTTTCATTAAGGTTCTAATG
GCTTTGGTTTATCCGGAGTTTAACAAACTTCTTACGTGAATCCATCAAACAAGATGGAAT
ATGTATAACTTCAGGGTTTGAAATAATACGAACATAACAAATATTTGGACCCACAAATAG
AACAAAACAACCAAAGAGTTTTTGTATATCAAACCTGGTTATCGTCGGCGTCACCCAAAG
CGATAGTGACCTGATCTCTAGACCAATCAGGAGAGAAACAGCTTGTGGTAACAATTACAG
TGTAAGGGCAGCTTTCTTCTTCATGAAGATCAAGAGAAGAGATTAGTGGCTCGAAGGTTA
GAGAAGGAATGGGATGAGTGAAGCAGAGTACAAGGAGAAGGAGGATGGATGAGAGAGAGC
ACTCCATTGT

Last Updated on 06.12.2018 16:11