Sequences from confirmation amplicon


Confirmation sequences for line 243E10 and hit At1g11960 are shown.
Line ID 243E10
Locus At1g11960 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:4042940
Primers used for PCR CP84 - 8474, show primer details
   
Sequence Name 51-K105459-0022-243-E10C-8409
GenBank Accession LN507501 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>51-K105459-0022-243-E10C-8409
ATTTACATTGAATTGAGCCTAAGTTAACATTGACAAACTTGCTGACAAGAGCACCTGAAT
GCAAAGGGCTGCTTCTTATACCTTTGAGATACCATTTAGGGAAATAAACCCTATCGTTGA
ATGGTTGAATCCTCAAGATTGCAAATGCCAAAAGGAAGATGATTGCAGTGAGTATGTTGA
TTGCTGCTGCTACTCCAATATCTCCTAGTGTTGCCATCTTTTTTTAACTCTTAGCTTAAA
TCTATTTATCAAATAAATGAATCTCCTTTGGGAATCAAACGCTTCTTCTTCACCTGCAAA
ATCAATTCGAACCCTTTGAAATCAAAAACTTCACCTACAAAAATATTCATTCAAAAAGCG
TGTGATATATGTACAGATTATGGAAATTTATATTTCTTAGATATAAAAAATTCTACATTT
TACCGAAAAGGAAATTGGTAAAGAGGAATTGG
   
Sequence Name 35-K105458-0022-243-E10C-CP84
GenBank Accession LN501832 [GenBank]
Sequencing primer gene specific primer, CP84: 5'-ACCAATTCCTCTTTACCAATTTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>35-K105458-0022-243-E10C-CP84
GAGTGATTTTATTCTAGAATAAAATTTCCATAATCTGTACATATATCACACGCTTTTTGA
ATGAATATTTTTGTAGGTGAAGTTTTTGATTTCAAAGGGTTCGAATTGATTTTGCAGGTG
AAGAAGAAGCGTTTGATTCCCAAAGGAGATTCATTTATTTGATAAATAGATTTAAGCTAA
GAGTTAAAAAAAGATGGCAACACTAGGAGATATTGGAGTAGCAGCAGCAATCAACATACT
CACTGCAATCATCTTCCTTTTGGCATTTGCAATCTTGAGGATTCAACCATTCAACGATAG
GGTTTATTTCCCTAAATGGTATCTCAAAGGTATAAGAAGCAGCCCTTTGCATTCAGGTGC
TCTTGTCAGCAAGTTTGTCAATGTTAACTTAGGCTCATATTCAATTGTAAATGGCTTCAT
GTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAG
AGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCACGGTTTATT

Last Updated on 06.12.2018 16:11