Sequences from confirmation amplicon


Confirmation sequences for line 243G06 and hit At3g58510 are shown.
Line ID 243G06
Locus At3g58510 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:21642898
Primers used for PCR GD33 - 8474, show primer details
   
Sequence Name 52-K100815-0022-243-G06C-8409
GenBank Accession LN507742 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>52-K100815-0022-243-G06C-8409
CCATTTACAATTGAATATCAATTGTGTCTGCCATGATGATCTTTAAAGAGTTCATTTCAG
TAATATCATCGAGGTTGTTTCTATAGTTTGGTTTTAGCTTTAGTGTGCACAATTATCCAA
ATTTCAACTTAACTCAATCAACGAACCTGTTGTGTTCTATCGCCATGAATGGAGGTTGCT
GGAAACTCATTCATGCACAGCCAATTTTCCAAAGTATCAGCTCCCCTCTTTGTCTCCACA
AAAACTAATGTCAATGATTGCTGTGTATAAAAAGTGAAGAAAGGCAGAATTAGTCAGAGC
TTCAGTGATAATAAATCTTGGATAAGTGAACAGATGGTAAATAATGCATTACCTTGTCTT
GGGTCTCTCTTTGCGCGTGAAGCAGGTCCATGAGATGGCTTCTCTTGTCAGACTCTTGGA
CAAACTCAACCCTTTGGGTAATT
   
Sequence Name 52-K100827-0022-243-G06C-GD33
GenBank Accession LN507744 [GenBank]
Sequencing primer gene specific primer, GD33: 5'-TAATTACCCAAAGGGTTGAGTTTG-3'
Sequence (A. th genome BLAST matches underlined) 
>52-K100827-0022-243-G06C-GD33
GACCTGCTTCCGCGCAAAGAGAGACCCAAGACAAGGTAATGCATTATTTACCATCTGTTC
ACTTATCCAAGATTTATTATCACTGAAGCTCTGACTAATTCTGCCTTTCTTCACTTTTTA
TACACAGCAATCATTGACATTAGTTTTTGTGGAGACAAAGAGGGGAGCTGATACTTTGGA
AAATTGGCTGTGCATGAATGAGTTTCCAGCAACCTCCATTCATGGCGATAGAACACAACA
GGTTCGTTGATTGAGTTAAGTTGAAATTTGGATAATTGTGCACACTAAAGCTAAAACCAA
ACTATAGAAACAACCTCGATGATATTACTGAAATGAACTCTTTAAAGATCATCATGGCAG
ACACAATTGATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAA
TGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCA
AAAATGTAGATGTCCGCACGGTTATT

Last Updated on 06.12.2018 16:11