Sequences from confirmation amplicon


Confirmation sequences for line 244A10 and hit At3g25870 are shown.
Line ID 244A10
Locus At3g25870 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:9464171
Primers used for PCR 15uv - 8474, show primer details
   
Sequence Name 73-K117671-0022-244-A10C-15uv
GenBank Accession LN512965 [GenBank]
Sequencing primer gene specific primer, 15uv: 5'-ACGTACGTACCGAATTTTAAACCA-3'
Sequence (A. th genome BLAST matches underlined) 
>73-K117671-0022-244-A10C-15uv
AAACGACATTTGAAATCCCAAAAATATAAAACCAAATAAAAATAAAAACCAGAAAAAAAA
AGAACTAGGAAAAAAAAAAGTGAAAGATCGGTGAGGCCCAAACTATTCGTTATACACGCG
CCAAAGTCGGCGTCGGAAATCACTCGCTTTCTCTTTCGTTACCGACTTTTATATCGGCTT
CTCTTTATATCTTCTCCCCACTATAAAATCTCGGGTTTCCCCTTCTTCCGATTCGGTACC
CATATCTCACGCTTAATTACAGTATTGCCACTCATTAAAACCGTACCATCTTAACCAAAG
CGATAACCCGTACCACCGGTTCAGCTATGGAGGCTCCATATGGACGGGATATATTCAATT
GTAAAGGGCTTCAGGTCCGGGAAATCTACAGGGATCACCAATGAGTATGAGGGTCAATAT
GGAGAAAAAGAAAGAGTAATTACCATTTTTTTTTCAATTCAAAAAGGTAGATGTCC
   
Sequence Name 25-K117670-0022-244-A10C-8409
GenBank Accession LN497638 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>25-K117670-0022-244-A10C-8409
TCAATTGAATTATCCTGTCAATATGGAGCCTCCATAGCTGAACCGGTGGTACGGGTTATC
GCTTTGGTTAAGATGGTACGGTTTTAATGAGTGGCAATACTGTAATTAAGCGTGAGATAT
GGGTACAGAATCGGAAGAAGGGGAAACACGAGATTTTATAGTGGAGAGAAGATATAAAGA
GAAGCAGATATAAAAGTCGGTAACGAAAGAGAAAGCGAGTGATTTCAGACGCAGACTTTG
GCGCGTGTATAACGAATAGTTTGGGCATCACAGATCTTTCACTTTTTTTTTTCCAAGTTC
TTTTTTTTTCGGGTTTTTATTTTTATTGGGTTTAATATTTTTGGGGATTTCAAATGTCGT
TTTCTAATTTCTGAACGTTATTATAAATTGGTTTAAAATTCGGTACGTAC

Last Updated on 06.12.2018 16:11