Sequences from confirmation amplicon


Confirmation sequences for line 245B08 and hit At5g17060 are shown.
Line ID 245B08
Locus At5g17060 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:5610826
Primers used for PCR JU30 - 8474, show primer details
   
Sequence Name 55-K024064-022-245-B08C-8409
GenBank Accession LN508683 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>55-K024064-022-245-B08C-8409
GTAGATTTCCCGGACATGAAGCCATTTACAATTGAATATTCCGTTGAAGAAGACTTGCTC
CTGAAATCCTTTGGACCGAAACTCGTAAAATTATTTCCCTTCAATTTCTCTGAAATCTAT
TCACCGTCTCCTTTGTCTCCTCCTCCAAATCGATCGTTCCTCGTCGTCGTCTGATTTTCT
CTACGATTGCTTTTTCTGTCTAGCTCGATAAGATAAAACAAAACTAATTTTTGAAGAGTT
TTTTTTTTTGGGTTTGGGAGCGATGGGTCAAGCTTTTCGTAAGCTATTCGATACTTTCTT
CGGCAATCAAGAAATGAGGGTAATCTCATCATCTCTCGGGCTATTTATCTGGATGGGATC
TCGGGTAATTTTGTCATCTTTGATTTGTTTTGGTTTTAGTCATAGCT
   
Sequence Name 56-K024064-022-245-B08C-JU30
GenBank Accession LN508983 [GenBank]
Sequencing primer gene specific primer, JU30: 5'-GGGCGATAAAAGCTATGACTAAAA-3'
Sequence (A. th genome BLAST matches underlined) 
>56-K024064-022-245-B08C-JU30
ACAAATCAAAGATGACAAAATTACACGAGATCCCATCCAGATAAATAGCACGAGAGATGA
TGAGATTACCCTCATTTCTTGATTGCCGAAGAAAGTATCGAATAGCTTACGAAAAGCTTG
ACCCATCGCTCCCAAACCCAAAAAAAAAAACTCTTCAAAAATTAGTTTTGTTTTATCTTA
TCGAGCTAGACAGAAAAAGCAATCGTAGAGAAAATCAGACGACGACGAGGAACGATCGAT
TTGGAGGAGGAGACAAAGGAGACGGTGAATAGATTTCAGAGAAATTGAAGGGAAATAATT
TTACGAGTTTCGGTCCAAAGGATTTCAGGAGCAAGTCTTCTTCAACGGAATATTCAATTG
TAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATG
GAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGC
GTTTTTATAAAATGAAAGTACTTTTTGATAAAACGACAAATTACGATCCGTCGTATTTAT
GGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCT

Last Updated on 06.12.2018 16:11