Sequences from confirmation amplicon


Confirmation sequences for line 245E05 and hit At1g03370 are shown.
Line ID 245E05
Locus At1g03370 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:832144
Primers used for PCR MF07 - 8474, show primer details
   
Sequence Name 68-K031692-022-245-E05C-8409
GenBank Accession LN511933 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>68-K031692-022-245-E05C-8409
CATTTACAATTGAATATATCCTGCATCAGCTTTACTGCATCTCTCCTGGGCCTGAGCTGC
CATCTGGTGAGCAGTGTTCACGTTTGGTAGTTTCATGGAGATTGAATTTTCTTCAGAGCA
CCATGATGAGAGGTATGATAGAGAATGGAGCTCGACAAGGTCTAAAAGACAACTTCGAAC
AGTATGCCAATTTGTTGGCTCAAAGTGTAAAGCCTGTAGATTCTAAGGATATTGGGCTGA
ACAAGGAGCAAGCTCTCTCTTCCTTGCAAGCTGAGCCTCAATCAGACTGGAAATTGGCTG
TACAGTATTTTGCCAATTTCACCGTGTTATCCACTTTCTTGATTGGTATTTATGTATTTG
TCCACATTGTGTTTGCCATACCCAGTGCAATCCAAGGGCTTGAGTTTAATGGCCTTGATT
TACCGGATTCAATTGGGGAGTTTGTTGTTAGTGGGGTTCTCGTTCTTCAATGTGAAAGAG
TATTGCAACTGATTTCCCGTTTCATGCAGGCAAGAAAACAAANAGGTAA
   
Sequence Name 20-K031692-022-245-E05C-MF07
GenBank Accession LN495097 [GenBank]
Sequencing primer gene specific primer, MF07: 5'-GTTACCTTTCTGTTTTCTTGCCTG-3'
Sequence (A. th genome BLAST matches underlined) 
>20-K031692-022-245-E05C-MF07
TCTTTCCATTGAAGACGAGAACCCACTAACAACAAACTCCCCAATTGAATCCGGTAAATC
AAGGCCATTAAACTCAAGCCCTTGGATTGCACTGGGTATGGCAAACACAATGTGGACAAA
TACATAAATACCAATCAAGAAAGTGGATAACACGGTGAAATTGGCAAAATACTGTACAGC
CAATTTCCAGTCTGATTGAGGCTCAGCTTGCAAGGAAGAGAGAGCTTGCTCCTTGTTCAG
CCCAATATCCTTAGAATCTACAGGCTTTACACTTTGAGCCAACAAATTGGCATACTGTTC
GAAGTTGTCTTTTAGACCTTGTCGAGCTCCATTCTCTATCATACCTCTCATCATGGTGCT
CTGAAGAAAATTCAATCTCCATGAAACTACCAAACGTGAACACTGCTCACCAGATGGCAG
CTCAGGCCCAGGAGAGATGCAGTAAAGCTGATGCAGGATATATTCAATTGTAAATGGCTT
CATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGA
AAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAA
AATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGC
AATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTACATTCACTCCCAA
ATG

Last Updated on 06.12.2018 16:11