Sequences from confirmation amplicon


Confirmation sequences for line 245H11 and hit At2g40095 are shown.
Line ID 245H11
Locus At2g40095 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:16743951
Primers used for PCR 50rq - 8474, show primer details
   
Sequence Name 69-K118051-0022-245-H11C-50rq
GenBank Accession LN512280 [GenBank]
Sequencing primer gene specific primer, 50rq: 5'-AACTATCGAGAGGACCGTATCTGA-3'
Sequence (A. th genome BLAST matches underlined) 
>69-K118051-0022-245-H11C-50rq
CTTCTCTATCACGTTTCGTTAAAGCTGCGTTTGTGTTCGGTGAGCCAGCGATCGGAGACG
GAGGAGGAAACTACTTCAGAGCGGCGGAAGTGGCTCTGTTTCTATGCTCGTCAGTCTTAG
CCATAGGTCACATCGTTGTGGCTTATCGAACAAGTTGTAGAGAAAGAAAAAAGCTTCTCG
TCTTCAAAATCGACATTGAAGCCGTAAGTCCACTTCTCCATCTTCTTTTTTTCCGCCGTT
ACAGAAATTTATTATTTTATTATATTTGATTGATAATCGATGTAGGGCCAATAATACCAA
GTTGCATTTACTTTTTTTCAAATCTAGGAATGAATTAGATTTTTAACCTGCAATGGGTCA
GAGTCAGAGATATATTCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTA
CATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATT
TTTTTTCAATTCAAAAATGTAGATGTCCG
   
Sequence Name 33-K118050-0022-245-H11C-8409
GenBank Accession LN501175 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>33-K118050-0022-245-H11C-8409
AACTCTGACCCATTGCAGGTTAAAAATCTAATTCATTCCTAGATTTGAAAAAAAGTAAAT
GCAACTTGGTATTATTGGCCCTACATCGATTATCAATCAAATATAATAAAATAATAAATT
TCTGTAACGGCGGAAAAAAAGAAGATGGAGAAGTGGACTTACGGCTTCAATGTCGATTTT
GAAGACGAGAAGCTTTTTTCTTTCTCTACAACTTGTTCGATAAGCCACAACGATGTGACC
TATGGCTAAGACTGACGAGCATAGAAACAGAGCCACTTCCGCCGCTCTGAAGTAGTTTCC
TCCTCCGTCTCCGATCGCTGGCTCACCGAACACAAACGCAGCTTTTAACGAAACGTAGAT
TAGAGAAGATATTGAACAAATCATTGTTATTGTCAGATACGGTCCT

Last Updated on 06.12.2018 16:11