Sequences from confirmation amplicon


Confirmation sequences for line 248A05 and hit At2g31750 are shown.
Line ID 248A05
Locus At2g31750 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:13497596
Primers used for PCR 22fr - 8474, show primer details
   
Sequence Name 33-K111470-0022-248-A05C-22fr
GenBank Accession LN501046 [GenBank]
Sequencing primer gene specific primer, 22fr: 5'-ACGCATTAAAGCAATTTAGCAAGT-3'
Sequence (A. th genome BLAST matches underlined) 
>33-K111470-0022-248-A05C-22fr
ATTTATACAGTATTTGCGAAATGCAACATCGTTAAGAAAAATATGAAATAAACTAATGAA
TTAAACCAAAAGAATAAAGGATAAAACTAAACTAATGAAACTAGAATTAAAAAATAAATC
AAATTGTTGTGCAAAATCTCAATTTGGTCAGGGACACATCCCCACATGCCTAGCCAGTAT
GTATGCTTTCAATGGAAAGGTAAACGGCGTGACACGTATGTATATTTGAGACGATATTCG
TTTTCTGGCTACATATTTGCCACGTTAAATTAAGACCCCAACCAATTGCATAAGATATAT
TATAAGTAATCTGCACCTTGGGATTTTCGCCCCAATCGATATATCTATATCTTTCGGCCC
CAAGATGAATGATGTTCTTTTTGTCCGTTGAAATAATGATGTGTTTTCTATCTAATATCT
TTTTGCTATTATTATGTACCAACATCGAATTAAAAAAATTTCACCACGAACCTAGAAAGC
TACAAGTCTAGCTACAACTATATTGGAATAAGGACTTATTTCACATCATATACTAATTGG
TTCAGTAAGATAGTAGTATATATCATCCACAATTATATCACCTCCAAATATTTTTATTTG
GATTGCATTTTGCAGATAATTAAGAAAAATACAATAAACAACAAAACTCTAAAGTTGAGA
ATTACCTCGACTTCGAGTTCGTCGAAAGAGTTAACCAAGAAGAAGTCAATGTCGTCAACA
TTCACGAACTGGCTACTAATGAGCTCAAACAACGGCCGGCAGAGATTGTTATCGTACAGA
AACACCGGTAAGTCATTACCCTTCAGCGGAGGCATTGCAGGCAAAACGACATCATTTTGA
AACTCCTTAAACTCTCCACGCAAGAAATGAATATAGGTCGCGTTCACGGTGGAGGACTGA
GTGAAAAACGACGCCGCAGCAACGCCAGGATGTTTCCGGCAAACGTCGAGGACATAAGGC
AGGCACGAGTCGTAAACGACGGCGTTTGGTTTTGGGTCCATCGAGGAGATAAGCTCTGAG
AGGCTT

Last Updated on 06.12.2018 16:11