Sequences from confirmation amplicon


Confirmation sequences for line 249B08 and hit At2g39300 are shown.
Line ID 249B08
Locus At2g39300 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:16411343
Primers used for PCR 15gp - 8474, show primer details
   
Sequence Name 26-K111730-0022-249-B08C-15gp
GenBank Accession LN497992 [GenBank]
Sequencing primer gene specific primer, 15gp: 5'-GGTAGGCTTAGCTTTTGAGGTTTT-3'
Sequence (A. th genome BLAST matches underlined) 
>26-K111730-0022-249-B08C-15gp
GGCTTCTACTAGGAGATATCAGACGGGTAAAGAATGATTGGGACTTGCTTTTAAGCGACT
AGAAAAAGAGAAGACTGAGTTACAAGTTCAGTTAGAGACAGAGCTTGATAGAAGGTCAAG
TGAGTGGACTTCGAAGGTCGAGAGTTTCAAGGTGGAGGAGAAAAGGCTGAGGGAACGTGT
CAGAGAGCTTGCTGAGCATAATGTCTCACTCCAGAGAGAAATATCGACCTTTCATGAAAA
GGAAACTGAGCGTATCGATATGATAAGACATTTGGATGAAACAGTTACGGAGTTGAGTGC
AACAGCAGAGGAAATGCGTGAAGAAAATTTGTTTCTTATGCAAAATCTCTCCAAGTTACA
AGAGAGTTACACTGGTAGTACCGATGATCTTGATTACGTAAGAAGAAACTTTGAAGAGAA
AGACATGGAATGCAAGGAGTTACATAAATCTGTTACAAGACTTCTAAGAACTTGCAAAGA
ACAAGAGTAATGAGTATGATGGTCATTGCTTCAGGATATATTCAATTGTAAATGGCTTCA
TGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAA
GAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCG
   
Sequence Name 10-K111729-0022-249-B08C-8409
GenBank Accession LN490720 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>10-K111729-0022-249-B08C-8409
ATTGAATATATCCTGAAGCAATGACCATCATACTCATTACTCTTGTTCTTTGCAAGTTCT
TAGAAGTCTTGTAACAGATTTATGTAACTCCTTGCATTCCATGTCTTTCTCTTCAAAGTT
TCTTCTTACGTAATCAAGATCATCGGTACTACCAGTGTAACTCTCTTGTAACTTGGAGAG
ATTTTGCATAAGAAACAAATTTTCTTCACGCATTTCCTCTGCTGTTGCACTCAACTCCGT
AACTGTTTCATCCAAATGTCTTATCATATCGATACGCTCAGTTTCCTTTTCATGAAAGGT
CGATATTTCTCTCTGGAGTGAGACATTATGCTCAGCAAGCTCTCTGACACGTTCCCTCAG
CCTTTTCTCCTCCACCTTGAAACTCTCGACCTTCGAAGTCCACTCACTTGACCTTCTATC
AAGCTCTGTCTCTAACTGAACTTGTAACTCAGTCTTCTCTTTTTCTAGTCGCTTTAAAAG
CAAGTCCCAATCATTCTTTACCCGTCTGATATCTTCCCTAGTAGAAGCCCTCTCATCCAT
TTGTGACCGTAAAAGACTTAAAACCTC

Last Updated on 06.12.2018 16:11