Sequences from confirmation amplicon


Confirmation sequences for line 250A05 and hit At2g13800 are shown.
Line ID 250A05
Locus At2g13800 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:5756088
Primers used for PCR JC25 - 8474, show primer details
   
Sequence Name 21-K101714-0022-250-A05C-8409
GenBank Accession LN495602 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>21-K101714-0022-250-A05C-8409
ATTTACAATTGAATATATCCTGATCATACTGATCATCTCAACTTCGGTTTGAAACTGCAG
TTCCCCACCCTTGGTACGTTCTTCATTTAGCCGTTTCACAGCCACTAGAGTGTCATCAGC
TAAACGTCCTTTATACAATATACCAAAACGTCCTTTGCCCAATACATTTCTTTTGCTAAA
TTTCTCTGTAGCAACTAGCAGTTCACGCAAGGAGAACCTTTTAAATTGTCCTAAATAAAC
CTCTGGGTCTTCTTCAGCTGCAAAATTTGGTTCACCAAAACGAGATTAAAATCAGCCGAA
GAAGCTTCAATGGGAAAATAAATGAAGAGAGTTGACTAACCAGGTACATCAAGAAAGTGA
CCCTGCAGTTTTCTTCTCAGCCACCAAGCAAGCGCAAATAGAAGTGCTGCACCCGCAGCA
ACTCCCACTACTATGGCTGCAGACGTTCCTGAGATAAACACA
   
Sequence Name 21-K101720-0022-250-A05C-JC25
GenBank Accession LN495603 [GenBank]
Sequencing primer gene specific primer, JC25: 5'-TTGTGTTTATCTCAGGAACGTCTG-3'
Sequence (A. th genome BLAST matches underlined) 
>21-K101720-0022-250-A05C-JC25
CACTTCTATTTGCGCTTGCTTGGTGGCTGAGAAGAAACCGCTGAGTCACTTTCTTGATGT
ACCTGGTTAGTCAACTCTCTTCATTTATTTTCCCATTGAAGCTTCTTCGGCTGATTTTAA
TCTCGTTTTGGTGAACCAAATTTTGCAGCTGAAGAAGACCCAGAGGTTTATTTAGGACAA
TTTAAAAGGTTCTCCTTGCGTGAACTGCTAGTTGCTACAGAGAAATTTAGCAAAAGAAAT
GTATTGGGCAAAGGACGTTTTGGTATATTGTATAAAGGACGTTTAGCTGATGACACTCTA
GTGGCTGTGAAACGGCTAAATGAAGAACGTACCAAGGGTGGGGAACTGCAGTTTCAAACC
GAAGTTGAGATGATCAGTATGATCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGG
AAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATT
ACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCACGGTTATT
Primers used for PCR JC26 - 8474, show primer details
   
Sequence Name 22-K101714-0022-250-A05C-OriL
GenBank Accession LN496053 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>22-K101714-0022-250-A05C-OriL
CATTTACAATTGAATATATCCTGAAAGATTACTTGTTTATCCCTACATGGCTAATGGAAG
TGTTGCTTCTTGTTTAAGAGGTACCACATTCTTTGTCCTAAAGGTTATTTGTCATATATT
TTTGTTTTGTTTTGACTGGTTTGTGATTGGTCAGTTGGTAAGTATCTTATCTTTGATTTT
CTATTATAGAGCGTCCTGAAGGCAATCCAGCCCTTGACTGGCCAAAAAGAAAGCATATTG
CTCTGGGATCAGCAAGGGGGCTCGCATATTTACACGATCATTGCGACCAAAAGATCATTC
ACCTGGATGTGAAAGCTGCAAATATACTGTTAGATGAAGAGTTTGAAGCTGTTGTTGGAG
ATTTTGGGCTAGCAAAATTAATGAATTATAACGACTCCCATGTG
   
Sequence Name 22-K101720-0022-250-A05C-JC26
GenBank Accession LN496054 [GenBank]
Sequencing primer gene specific primer, JC26: 5'-TCACATGGGAGTCGTTATAATTCA-3'
Sequence (A. th genome BLAST matches underlined) 
>22-K101720-0022-250-A05C-JC26
ACACAGCTTCAACTCTTCATCTAACAGTATATTTGCAGCTTTCACCCCTGGTGAATGATC
TTTTGGTCGCAATGATCGTGTAAATATGCGAGCCCCCTTGCTGATCCCAGAGCAATATGC
TTTCTTTTTGGCCAGTCAAGGGCTGGATTGCCTTCAGGACGCTCTATAATAGAAAATCAA
AGATAAGATACTTACCAACTGACCAATCACAAACCAGTCAAAACAAAACAAAAATATATG
ACAAATAACCTTTAGGACAAAGAATGTGGTACCTCTTAAACAAGAAGCAACACTTCCATT
AGCCATGTAGGGATAAACAAGTAATCTTTCAGGATATATTCAATTGTAAATGGCTTCATG
TCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGA
GTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCG

Last Updated on 06.12.2018 16:11