Sequences from confirmation amplicon


Confirmation sequences for line 250D01 and hit At4g16090 are shown.
Line ID 250D01
Locus At4g16090 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:9103370
Primers used for PCR KB26 - 8474, show primer details
   
Sequence Name 61-K100815-0022-250-D01C-8409
GenBank Accession LN510454 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>61-K100815-0022-250-D01C-8409
CCATTTACATTTACAAGGTATTACCAATGGAGTTGCGTAAAGGGTGTCTGGTCGGAATGT
CCCATGGTTGGGGAGTTTTCAAGGCCGTGTGTGATTATAATGAATATAAAGCCATATATG
TTACTGACTACTACAATCCATGTGGCTCCAAATCTGTCAAAAGCATTCCTTTGCATCCCC
TTGGTAAACCTATAGCTATCCAACAACAAGCTATCACTAACGCCGCAATGACTTGTTCAC
CTGATCAAAGCAAAGATTTTGCAGTTGCTGTCAAGTGTTTAGGACCTATGATCAATTTCA
ATAGGCCTGGTGGCACACACAAGGACTCGGGTTCGGTCATCTTTAAAACCCCTTTTCAAT
ATTTTGATCAATCAAAGGTTATGTATTCAAAGAGAGATGAAAAGTTCTACACTCCTAGTT
TTGGAGGGCACTTCTTGGTTTTTTGGGATTCTTTCTTCGAGAAGGATA
   
Sequence Name 61-K100827-0022-250-D01C-KB26
GenBank Accession LN510456 [GenBank]
Sequencing primer gene specific primer, KB26: 5'-ATATCCTCTCCGAAGAAAGAATCC-3'
Sequence (A. th genome BLAST matches underlined) 
>61-K100827-0022-250-D01C-KB26
AACTTTTCATCTCTCTTTGAATCATAACCTTTGATTGATCAAAATATTGAAAAGGGGTTT
TAAAGATGACCGAACCCGAGTCCTTGTGTGTGCCACCAGGCCTATTGAAATTGATCATAG
GTCCTAAACACTTGACAGCAACTGCAAAATCTTTGCTTTGATCAGGTGAACAAGTCATTG
CGGCGTTAGTGATAGCTTGTTGTTGGATAGCTATAGGTTTACCAAGGGGATGCAAAGGAA
TGCTTTTGACAGATTTGGAGCCACATGGATTGTAGTAGTCAGTAACATATATGGCTTTAT
ATTCATTATAATCACACACGGCCTTGAAAACTCCCCAACCATGGGACATTCCGACCAGAC
ACCCTTTACGCAACTCCATTGGTAATACCTTGTAAATTGTAAATGGCTTCATGTCCGGGA
AATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTA
CCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGC

Last Updated on 06.12.2018 16:11