Sequences from confirmation amplicon


Confirmation sequences for line 250D07 and hit At3g18040 are shown.
Line ID 250D07
Locus At3g18040 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:6177334
Primers used for PCR DL91 - 8474, show primer details
   
Sequence Name 19-K018911-022-250-D07C-DL91
GenBank Accession LN494560 [GenBank]
Sequencing primer gene specific primer, DL91: 5'-TTGTTGCAGATTCGTAATGAAAAG-3'
Sequence (A. th genome BLAST matches underlined) 
>19-K018911-022-250-D07C-DL91
GATATCTCGGCAATATGAGGAGAAAACCGCCAGTTCCATTCACTCACAAATTTCCTCATG
TAGACCCATTGGCCCTTCGCTTACTGCACCGTCTTCTTGCATTTGATCCCAAAGACCGTC
CCTCAGCTGAAGAGGTTAGCTCCTCTATACTTGTTTTTTCTAGCGTTTCCACTTCAAAAT
TTCAGAGCAGTTTCAATCGCTCTCAGAAAAGGAAACATTATATATACATGTGTGTGTGTA
TATACAGGCACTGGCAGATCCTTACTTCTATGGTCTGGCAAACGTTGATCGGGAACCATC
TACTCAACCGATTCCGAAACTCGAGTTTGAGTTTGAAAGAAGGAAGATAACAAAAGAAGA
TGTGAGAGAATTGATCTACAGAGAGGTACACTAATCTCTTACTGTTTTTGCTTCACTAAA
CTTGTTTGGATTGATTAATATGATGCATGGACAGGATATATTCAATTGTAAATGGCTTCA
TGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAA
GAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAA
TGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAA
TAAACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTACAT
   
Sequence Name 23-K018911-022-250-D07C-8409
GenBank Accession LN496363 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>23-K018911-022-250-D07C-8409
GTAGATTTCCCGGACATGAAGCCATTTACAATTGAATATATCCTGTCCATGCATCATATT
AATCAATCCAAACAAGTTTAGTGAAGCAAAAACAGTAAGAGATTAGTGTACCTCTCTGTA
GATCAATTCTCTCACATCTTCTTTTGTTATCTTCCTTCTTTCAAACTCAAACTCGAGTTT
CGGAATCGGTTGAGTAGATGGTTCCCGATCAACGTTTGCCAGACCATAGAAGTAAGGATC
TGCCAGTGCCTGTATATACACACACACATGTATATATAATGTTTCCTTTTCTGAGAGCGA
TTGAAACTGCTCTGAAATTTTGAAGTGGAAACGCTAGAAAAAACAAGTATAGAGGAGCTA
ACCTCTTCAGCTGAGGGACGGTCTTTGGGATCAAATGCAAGAAGACGGTGCAGTAAGCGA
AGGGCCAATGGGTCTACATGAGGAAATTTGTGAGTGAATGGAACTGGCGGTTTTCTCCTC
ATATTGCCGAGATATCTCCTCGCCTTTTCATTACGAA
Primers used for PCR 13ah - 8474, show primer details
   
Sequence Name 48-K118837-0022-250-D07C-13ah
GenBank Accession LN506651 [GenBank]
Sequencing primer gene specific primer, 13ah: 5'-AGAATCCGGGAAATCAGTTTTACT-3'
Sequence (A. th genome BLAST matches underlined) 
>48-K118837-0022-250-D07C-13ah
CTAGGCAGGAAGCGTGTTGGCGTTGAAGAGGTGAGCCTTTCTCACCCTTACCGTAATTTT
CCTCAAGATGAGCAAATTGTCTCTTAAAGCGATCAACACCACTGCATTTTCATTTAGATT
ACAAAATCTTTATCTTCACTTCTCATAATTCGTAGTAAATTTTGAACACGGATTCTAAAT
GTTTTTATATACCTTGGGTACATGAAGCTAGTCTGTTCTCCACCTCGAAGATATTCTTGC
AGCATCTGAGGATGATACTCTAATATCTGCATCACATTCAACTTTTGTAAGGATTACGAT
TACCCGATGGATACGTGCGAAAAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAA
ATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTAC
CAATTTTTTTTCAATTCAAAAATGTAGATGTCCGACACGTTATTATAAT
   
Sequence Name 24-K118836-0022-250-D07C-8409
GenBank Accession LN497205 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>24-K118836-0022-250-D07C-8409
TCATTGAATATATCCTTTTCGCCGTATCCATCGGGTAATCGTAATCCTTACAAAAGTTGA
ATGTGATGCAGATATTAGAGTATCATCCTCAGATGCTGCAAGAATATCTTCGAGGTGGAG
AACAGACTAGCTTCATGTACCCAAGGTATATAAAAACATTTAGAATCCGTGTTCAAAATT
TACTACGAATTATGAGAAGTGAAGATAAAGATTTTGTAATCTAAATGAAAATGCAGTGGT
GTTGATCGCTTTAAGAGACAATTTGCTCATCTTGAGGAAAATTACGGTAAGGGTGAGAAA
GGCTCACCTCTTCAACGCCAACACGCTTCCTTGCCTAGGTATACAATTTCGTTTGTTGGT
TTGTCAGTAAAACTGATTTCCACGGATTC

Last Updated on 06.12.2018 16:11