Genotyping and primer details


Genotyping and primer details for 250D07 - At3g18040
Line ID 250D07
Locus At3g18040 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:6177334 of line 250D07 Chr3:6177334
   
Insertion allele amplicon 8474 - 13ah
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 13ah: 5'-AGAATCCGGGAAATCAGTTTTACT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:6177708
   
Insertion allele amplicon 8474 - DL91
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer DL91: 5'-TTGTTGCAGATTCGTAATGAAAAG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:6176832
   
wt amplicon (over InsPos) 13ah - DL91
wt amplicon start Chr3:6176832
wt amplicon end Chr3:6177708
wt amplicon size 877 bp
Locus specific primer DL91: 5'-TTGTTGCAGATTCGTAATGAAAAG-3'
2nd locus specific primer 13ah: 5'-AGAATCCGGGAAATCAGTTTTACT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11