Sequences from confirmation amplicon


Confirmation sequences for line 250D10 and hit At1g29080 are shown.
Line ID 250D10
Locus At1g29080 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:10157901
Primers used for PCR GG04 - 8474, show primer details
   
Sequence Name 22-K100938-0022-250-D10C-8409
GenBank Accession LN496036 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>22-K100938-0022-250-D10C-8409
GAGCCATTTACATTGAATATATCCTGACGAAAAATGCCTCGGTGTTTAATTATGTAGTTG
AAAGCATTTACAAATGTTCCTCCCTTGCAACCATTGTTCTGCTCTCTGGTGCAATCTAGA
AGTTGTTGTTCGGATAGTGATATGAGGTTTCCACGGGCAATCTTTGTCAGACCTTCCACC
GCTGCTATCGCTGAGAATGCCCAACAACCTCCTGCTCATATATATTTATAGGTTACGTGA
GGAATAAGTTTTCTAAATACGGAGTGTAATTATCCAGCCGTTTCATACATTACATTGCTT
CCAAATTTCATACATGTAGAGACAGTTTATATATAAAGATGACAAGCTTACCACATTCTC
CTTGGGATTTCACAGGTGTTACGGCTCCTTCGTTTCTCCAATCTTTGTTCGTGCCAAGGA
CATCACTGACGGTCCAATTCCAAGCTGGCTTCGTTTCATTAACCACTTCGAATGGTGAAG
TTACATTAACGCCTCTAAGACCGGTGTAGGTGGCAAGGAACTCCTCTTTAGTCCAATCTG
TGAACTCGTTAACACCAAGTTTGTAGCTNTGATTCCCCATAT
   
Sequence Name 22-K100944-0022-250-D10C-GG04
GenBank Accession LN496037 [GenBank]
Sequencing primer gene specific primer, GG04: 5'-GAGCTTCAATAATATGGGGAATCA-3'
Sequence (A. th genome BLAST matches underlined) 
>22-K100944-0022-250-D10C-GG04
GTTCACAGATTGGACTAAAGAGGAGTTCCTTGCCACCTACACCGGTCTCAGAGGCGTTAA
TGTAACTTCACCATTCGAAGTGGTTAATGAAACGAAGCCAGCTTGGAATTGGACCGTCAG
TGATGTCCTTGGCACGAACAAAGATTGGAGAAACGAAGGAGCCGTAACACCTGTGAAATC
CCAAGGAGAATGTGGTAAGCTTGTCATCTTTATATATAAACTGTCTCTACATGTATGAAA
TTTGGAAGCAATGTAATGTATGAAACGGCTGGATAATTACACTCCGTATTTAGAAAACTT
ATTCCTCACGTAACCTATAAATATATATGAGCAGGAGGTTGTTGGGCATTCTCAGCGATA
GCAGCGGTGGAAGGTCTGACAAAGATTGCCCGTGGAAACCTCATATCACTATCCGAACAA
CAACTTCTAGATTGCACCAGAGAGCAGAACAATGGTTGCAAGGGAGGAACATTTGTAAAT
GCTTTCAACTACATAATTAAACACCGAGGCATTTCTTCGTCAGGATATATTCAATTGTAA
ATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAG
AAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCANAAATGTAGATTC

Last Updated on 06.12.2018 16:11