Sequences from confirmation amplicon


Confirmation sequences for line 250F04 and hit At2g44790 are shown.
Line ID 250F04
Locus At2g44790 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:18462993
Primers used for PCR 14wc - 8474, show primer details
   
Sequence Name 44-K118511-0022-250-F04C-14wc
GenBank Accession LN505341 [GenBank]
Sequencing primer gene specific primer, 14wc: 5'-TGGGAAGAACCATACTTGAACTCT-3'
Sequence (A. th genome BLAST matches underlined) 
>44-K118511-0022-250-F04C-14wc
TTAAACCTAAGTAAGAGTTCTCCATACCTAGGAAGATTATAAGACAAGAGTCCCAAATTA
TCTGGTTACCAAATCTGAGATTTCTTGTAGAATAAGTTATCTCAATTTCAACATGGTAGA
AATTTATAAAGAAGTAAAACAAGCAAATCAAAAAATTGTTTCTTCTTCTTTCTTTTTACT
GTTTCTAACTTTCTATATTTAAGAACCTGAGACCAAATAAACAAAAAAAAAATCACACAT
TTTTTTTTTTTGGGTTTCCCTACAAAAGCAAACTTAATTTATTTTGAACCCCCCAAACCA
AATTTAAACCACCCTGGGGTTAAAATAGGGTACCACCCTTTTTGGGGGAAAGCTGGAATC
CTTTTAAAAATTTAAAAATTTTTAAAAAAGGGGGTTCGGAAAAAAATTTCCCCAAAAGGG
CCCCCACCCTGAAAGTTTTTCCGGGGCCCCCACCCGGGTTTGAATCCCATTGAAATTTTT
AGGGCAATTGGAAAGGGGTTCCTGGCCGGGAAATTTCCCGGGATCCCCAAGGGATTTAAG
GGGCAATTTGGGAAAAAAAAAAAAATATTTCCCCTTTTTTTTTCAATTCAAAAAGGGAAA
TTCCCC
   
Sequence Name 16-K118510-0022-250-F04C-8409
GenBank Accession LN493633 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>16-K118510-0022-250-F04C-8409
ATTTACATTGACATGATATATTCAATTGTACTCATACTCAGGTTGGGCTACCGGAAAGAC
TTTCAGGGTTGGTGACATTCTAGGTAAATCATCATCCGAACCACATTACTTATAACTTTT
ATAATTTTCAGATAGATTCAAGCTATACATCAAAAAGTGTAGTAACCTAATCTAACCCAA
GTGTAGTTTAATATTGGTTTCGGTGGTTCAAATTAAATTAAGTTTGCTTTAGTACGTAAT
ACCAAAAAAAAAAAAGGGGGGAATTTTTTTTTGTTTTTTGGGGCCCGGGGTCTTAAAATA
AAAAAGTTAAAAACCGTAAAAAAAAAAAAAAAAAAACCATTTTTTGATTTGGTGGGTTTA
CCTTTTTAAAAATTTCCCCCCGGGTGAAATTGGAAAAACTTTTTCCAAAAAAAATTCCAA
ATTTGGGAACCAAAAAATTGGGGACCCTGGGCTTAAAACCCTCCCGGGGGGGGAAAACCT
TTTACCTGGGGTTTAATTTTTTTTTTTTGGGGGAATTAAAAAAAAATTCAAGTTGGGTGT
TTTCCAAAAAAAA

Last Updated on 06.12.2018 16:11