Sequences from confirmation amplicon


Confirmation sequences for line 251D02 and hit At1g14390 are shown.
Line ID 251D02
Locus At1g14390 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:4925939
Primers used for PCR PP16 - 8474, show primer details
   
Sequence Name 11-K102723-0022-251-D02C-8409
GenBank Accession LN491063 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>11-K102723-0022-251-D02C-8409
TCGGACTGAGCATTTACAATTGCTGATCCAGAACACTGACCAAATGCATATGCCTTATCT
TTGATAAAACTTCCATTTGTTGAGCTAAATTCTGTGTTGAATTCTTCTGTTTCAGCTTAA
TACACCTCACTGTCACTGCTATGCCTTCTCTAAGACAACCTTTGTACAGCTGTTTTGAAA
TTAGATATATCGATTAGTTAGACACTCGGATCAATTTTAAATCATGTCTTATTTACATTT
TCCTTTACCTGTTCTCCACAGAGATTCTCTGCATCAAAATTGTTGGTTGCTTCTTCCAGT
TCCTCCAAAGAGAAAACTCGGTATGGTGATAGTCCAATCACCGCGGATCTCATGGTTTGC
GGTACACGTCCTGCATTTGAAAAAATCAAGCTAGTTAGGTTAATGGGTGTGAAAACTTGA
TTCAAGATTTCAAAGTAATAGCCTACTTAGATCTGGTACTGTCTTTGATGTGGTGGACCT
GGTGGTGTTGCTGCAAACAGTGACCTGATCGACGTTGTTTGCTTCCAAAGGCTCTTCTTT
TGATCTTGATTTTCTCATCCTAACCAAAACCAAGCCAGCTAAAACCGCTGAAACGAGGAT
CACACCGATGATAATGCCAATCACCAATCCAAGTTCGATTCCTGTATCTTCTTCTTTCCT
TTCTTTATCTTTAGTGTCAGACTTCACAGCAGCTACTGCTTGCTTTGCTTCGTTTTCACA
GAAGGTAACTGGACGCTGATACTTTG
   
Sequence Name 27-K102728-0022-251-D02C-PP16
GenBank Accession LN498336 [GenBank]
Sequencing primer gene specific primer, PP16: 5'-GGTTCTCCTTCTGCAAAGTATCAG-3'
Sequence (A. th genome BLAST matches underlined) 
>27-K102728-0022-251-D02C-PP16
GAGCAAGCAAGCAGTAGCTGCTGTGAAGTCTGACACTAAAGATAAAGACAGGAAAGAAGA
AGATACAGGAATCGAACTTGGATTGGTGATTGGCATTATCATCGGTGTGATCCTCGTTTC
AGCGGTTTTAGCTGGCTTGGTTTTGGTTAGGATGAGAAAATCAAGATCAAAAGAAGAGCC
TTTGGAAGCAAACAACGTCGATCAGGTCACTGTTTGCAGCAACACCACCAGGTCCACCAC
ATCAAAGACAGTACCAGATCTAAGTAGGCTATTACTTTGAAATCTTGAATCAAGTTTTCA
CACCCATTAACCTAACTAGCTTGATTTTTTCAAATGCAGGACGTGTACCGCAAACCATGA
GATCCGCGGTGATTGGACTATCACCATACCGAGTTTTCTCTTTGGAGGAACTGGAAGAAG
CAACCAACAATTTTGATGCAGAGAATCTCTGTGGAGAACAGGTAAAGGAAAATGTAAATA
AGACATGATTTAAAATTGATCCGAGTGTCTAACTAATCGATATATCTAATTTCAAAACAG
CTGTACAAAGGTTGTCTTAGAGAAGGCATAGCAGTGACAGTGAGGTGTATTAAGCTGAAA
CAGAAGAATTCAACACAGAATTTAGCTCAACAAATGGAAGTTTTATCAAAGCTAAGGCAT
ATGCATTTGGTCAGTGTTCTTGGATCAGCAATTGTAAATGGCTTCATGTCCGGGAAATCT
ACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAAT
TTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTT
GATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTC
TAATTCGGAAATCTTTATTTCGACGTGTCTACA
Primers used for PCR KC87 - 8474, show primer details
   
Sequence Name 41-K118534-0022-251-D02C-KC87
GenBank Accession LN504261 [GenBank]
Sequencing primer gene specific primer, KC87: 5'-CTCTATTTCCAAATTGTTCCCAAA-3'
Sequence (A. th genome BLAST matches underlined) 
>41-K118534-0022-251-D02C-KC87
TCGAGCACTCCAATCGCTATCGACATTCTCTGAGGCCATTTCAACACNCTCTTTCTTTCT
CCAATCTGCCAAAAACATTTCCAAATAATTAGTTTCCCAACAAAAATATAAGTTCTTGAA
ATCGATAATCTTTTTCAAGTATTACCGGTGAGGTAATCCCTCAAAGACCCGTTAGAGATA
TATTCTTGGACAATGAAAATAGTGCTTCCGGCATAAGGATGATGATCTTGATAAGTACCA
ATGCATATACCTTAGCTTCAAATGCATATATTCAATTGTAAATGGCTTCATGTCCGGGAA
ATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTAC
CAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCACCGATTATTAT
   
Sequence Name 20-K118533-0022-251-D02C-8409
GenBank Accession LN495424 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>20-K118533-0022-251-D02C-8409
ATTTCATTGAATATATGCATTTGAAGCTAAGGTATATGCATTGGTACTTATCAAGATCAT
CATCCTTATGCCGGAAGCACTATTTTCATTGTCCAAGAATATATCTCTAACGGGTCTTTG
AGGGATTACCTCACCGGTAATACTTGAAAAAGATTATCGATTTCAAGAACTTATATTTTT
GTTGGGAAACTAATTATTTGGAAATGTTTTTGGCAGATTGGAGAAAGAAAGAGGTGTTGA
AATGGCCTCAGAGAATGTCGATAGCGATTGGAGTTGCTCGAGGAATACAGTTCTTGCACA
CAGGAGTGGCACCAGGAATATTTGGGAACAATTTGGAAAATAGA

Last Updated on 06.12.2018 16:11