Genotyping and primer details


Genotyping and primer details for 251D02 - At1g14390
Line ID 251D02
Locus At1g14390 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:4925939 of line 251D02 Chr1:4925939
   
Insertion allele amplicon 8474 - KC87
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer KC87: 5'-CTCTATTTCCAAATTGTTCCCAAA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:4926254
   
Insertion allele amplicon 8474 - PP16
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer PP16: 5'-GGTTCTCCTTCTGCAAAGTATCAG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:4925210
   
wt amplicon (over InsPos) KC87 - PP16
wt amplicon start Chr1:4925210
wt amplicon end Chr1:4926254
wt amplicon size 1045 bp
Locus specific primer PP16: 5'-GGTTCTCCTTCTGCAAAGTATCAG-3'
2nd locus specific primer KC87: 5'-CTCTATTTCCAAATTGTTCCCAAA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11