Sequences from confirmation amplicon


Confirmation sequences for line 251D04 and hit At5g02080 are shown.
Line ID 251D04
Locus At5g02080 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:411235
Primers used for PCR 44xy - 8474, show primer details
   
Sequence Name 34-K119596-0022-251-D04C-44xy
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer gene specific primer, 44xy: 5'-ACTGCCATAAAGCGTAGTGAGAAT-3'
Sequence (A. th genome BLAST matches underlined) 
>34-K119596-0022-251-D04C-44xy
TTCTTTCTTTTTGTGATGTTTCTGGTTACAGATGTTGCGGTTGATTGCAGAGGCATTGAA
AGATGTTGGTCCATGTTCAATGTTTTATCTTGCTGCTGCTGTATCTGACTTCTATGTGCC
ATGGAAGAGCATGGTACGAGTCTCATGTCCCTAAGACTATGCTACACAATTCTTGTATCT
GAAAGATGTTTTTTGTATATATTTGGTGAAACCAGACAGAGCACAAAATAGAATCAGGAT
CTGGTCCTTTAGACATAAGACTAGCTCAAGTCCCTAAAATGCTATCAGTCTTGAGATCAA
ATTGGGCTCCAAAGGCCTTCTGCATATCATTCAAGTCCGGGAAATCTACATGGATCAGCA
ATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAG
AAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCACGGTT
ATT
   
Sequence Name 10-K119595-0022-251-D04C-8409
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>10-K119595-0022-251-D04C-8409
CATTGCTGATCCATGTAGATTTCCCGGACTTGAATGATATGCAGAAGGCCTTTGGAGCCC
AATTTGATCTCAAGACTGATAGCATTTTAGGGACTTGAGCTAGTCTTATGTCTAAAGGAC
CAGATCCTGATTCTATTTTGTGCTCTGTCTGGTTTCACCAAATATATACAAAAAACATCT
TTCAGATACAAGAATTGTGTAGCATAGTCTTAGGGACATGAGACTCGTACCATGCTCTTC
CATGGCACATAGAAGTCAGATACAGCAGCAGCAAGATAAAACATTGAACATGGACCAACA
TCTTTCAATGCCTCTGCAATCAACCGCAACATCTGTAACCAGAAACATTCACAAAAAGAA
AGTAATTTCAGCCATGGCAGCTCTGTTGAAGAATTCTCACTACGCTTTATGGCAG

Last Updated on 06.12.2018 16:11