SimpleSearch - Line and FST details


Line specific information

 
Line ID 251D04
Vector Used pAC161
Line Availability available as T3 set from NASC (N424040)
Segregation Analysis N/A (line seemingly not resistant, T2 plants grown without drug)
Confirmed for Hit At5g02080
Parent of DUPLO pair 12088
Parent of pair(s) 12282
Note

Gene hit At2g19350

 
Sequence (A. th genome BLAST matches underlined) 
>28-K014555-022-251-D04-8409
TTGATCCATTGTAGATTTCCCGCACATGAAGCACTTGACGATCGATATATATCGACCCGT
ACTTGAATGATCTGCGGAAGGCCTTCGGAGCCCAATTTGATCTCAAGACTGATAGCATTT
TAGGGACTTGAGCTATGATATACTGTTCCTCCATATGTCAACATATTTTATTGCCTATGG
GATCCTCCCTATAGTGAGAGAGTTGTACTACTTTTTCTCAATCTAAGCTAATCACGAAGA
CACAAGGTCAAAATTACTAACAATTTTGATTAATAAGGAATCGCAATTAAAACCGAGACC
TCGATCACCACCAAGACGGTTATACGCCATCAAAAAACCTAATACAATATCTATGGGATC
CTCCCTATAGTGAG
GenBank Accession FR806521 [GenBank]
Graphic View Graphic view of gene At2g19350
Predicted Position of Insertion Chr2:8376753 - go to primer design
BLAST e Value 2e-43
Hit Clone Code (BAC ID) F27F23
Hit Gene Code At2g19350 [Araport] [TAIR] [MIPS] [SIGnAL]
Gene Annotation transmembrane protein (DUF872)
Insertion Classification CDSi
Confirmation Status failed

Gene hit At5g02080

 
Sequence (A. th genome BLAST matches underlined) 
>28-K014552-022-251-D04-8409
NTGATCCATGTAGATTNTCCCGGACATGAAGCACTTGCTGATCCATGTAGATTTCCCGGA
CTTGAATGATATGCAGAAGGCCTTTGGAGCCCAATTTGATCTCAAGACTGATAGCATTTT
AGGGACTTGAGCTATGGGATCCTCCCTATAGTGAG
GenBank Accession AL940752 [GenBank]
Graphic View Graphic view of gene At5g02080
Predicted Position of Insertion Chr5:411235 - go to primer design
BLAST e Value 5e-34
Hit Clone Code (BAC ID) T7H20
Hit Gene Code At5g02080 [Araport] [TAIR] [MIPS] [SIGnAL]
Gene Annotation phosphopantothenate-cysteine ligase-like protein
Insertion Classification CDSi
Confirmation Status confirmed, show confirmation sequences
Primer and wt-amplicons show primer details


Last Updated on 10.06.2021 13:37