Sequences from confirmation amplicon


Confirmation sequences for line 251G03 and hit At2g38220 are shown.
Line ID 251G03
Locus At2g38220 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:16008885
Primers used for PCR PA54 - 8474, show primer details
   
Sequence Name 43-K037018-022-251-G03C-8409
GenBank Accession LN504759 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>43-K037018-022-251-G03C-8409
CATTTCAATTGAATAATCCTGAGAGAAGCAAAATCAGTATGGCCCTATGAATATATCCCG
GACATGAAGCCATTTACAATTGAATATATCCTGAGAGAAGCCATTTACTCAGTGATCTAT
TTAAGTAGTATGGCCATCCCTGAGAGAAGCAAAATCAGTTCAAAAAAATCAGTCTAAAAG
AGACTTCAACGACAATAATCAAAGCTGAAAATAAATTCTGACAAAATATATAGACAAAGA
TCAAATCTTTGGTTTCAATGGCAAGTACCCAAAACAATACAATAAAGATCCTAAGGAATT
TTAAACCTGCCTTGTAAGAATTCTGTGAGACAGATAACACTCGAGATTCAGTCCAGTTTA
CAAAATAACCACTAGGAGTAGATTGACCATTGAATCCATATAGCTGAAGTCCAGGCTTTG
AAAAATCAAGCTCCTCAACCTAAC
   
Sequence Name 44-K037018-022-251-G03C-PA54
GenBank Accession LN505101 [GenBank]
Sequencing primer gene specific primer, PA54: 5'-TGTTAGGTGAAGGAGCTTGATTTT-3'
Sequence (A. th genome BLAST matches underlined) 
>44-K037018-022-251-G03C-PA54
GGATTCATGGTCATCTACTCCTAGTGGTTATTTTGTAAACTGGACTGAATCTCGAGTGTT
ATCTGTCTCACAGAATTCTTACAAGGCAGGTTTAAAATTCCTTAGGATCTTTATTGTATT
GTTTTGGGTACTTGCCATTGAAACCAAAGATTTGATCTTTGTCTATATATTTTGTCAGAA
TTTATTTTCAGCTTTGATTATTGTCGTTGAAGTCTCTTTTAGACTGATTTTTTTGAACTG
ATTTTGCTTCTCTCAGGGATGGCCATACTACTTAAATAGATCACTGAGTAAATGGCTTCT
CTCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGATATATTCATATGGCCATACT
GATTTTGCTTCTCTCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACA
TGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTT
TTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGAT
AAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAA
TTCGGAAATCTTTATTTCGACGTGTCTACATTCACT

Last Updated on 06.12.2018 16:11