Sequences from confirmation amplicon


Confirmation sequences for line 252E04 and hit At3g63260 are shown.
Line ID 252E04
Locus At3g63260 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:23373955
Primers used for PCR CP96 - 8474, show primer details
   
Sequence Name 81-K105358-0022-252-E04C-CP96
GenBank Accession LN514072 [GenBank]
Sequencing primer gene specific primer, CP96: 5'-AGATTGACCTCTCCAAACTCGATA-3'
Sequence (A. th genome BLAST matches underlined) 
>81-K105358-0022-252-E04C-CP96
TTACGGCACTGTCTCCGCGGTGTCTACGCCGGCCAAGAAGTCGCAGGTCCGTCTTTTCTC
CTAGACTAAGATATATATGATTCATTAGGATAGACACTGGTGGTCTTTTTTGGGGATCTC
CCGTCTGATGGATGTTATGGTTTGCTGCAGTGAAAGTGTTAGATTGGGGAGAAGATGGTT
ACGCCACACCAGCTGAAACTACAGCTCTCCGTGCTTCCTTCGAGCAAGAGGTCGCCGTCT
GGCAGAAGCTCGATCATCCCAACGTTACCAAGGTATTCTTTTTACATTCACCGATTAAGG
TAGAAGAAGAACCTTTTGATCAAAGGACTTATTCATTTGACCTCTCTCTCTCTGTGGCAG
TTTATAGGAGCATCCATGGGAACCTCTGATCTGCGGATCCCTCCTGCTGGTGATACTGGC
GGACGTGGTAACGGTGCACATCCTGCGAGGGCCTGTTGTGTTGTGGTTGAATATGTTGCC
GGAGGCACGCTTAAGAAGTTCCTCATCAAGAAATATATTCAATTGTAAATGGCTTCATGT
CCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAG
TAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCACGGGTTATT
   
Sequence Name 65-K105359-0022-252-E04C-8409
GenBank Accession LN511401 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>65-K105359-0022-252-E04C-8409
TTTCATTGAATAATTTCTTGATGAGGAACTTCTTAAGCGTGCCTCCGGCAACATATTCAA
CCACAACACAACAGGCCCTCGCAGGATGTGCACCGTTACCACGTCCGCCAGTATCACCAG
CAGGAGGGATCCGCAGATCAGAGGTTCCCATGGATGCTCCTATAAACTGCCACAGAGAGA
GAGAGGTCAAATGAATAAGTCCTTTGATCAAAAGGTTCTTCTTCTACCTTAATCGGTGAA
TGTAAAAAGAATACCTTGGTAACGTTGGGATGATCGAGCTTCTGCCAGACGGCGACCTCT
TGCTCGAAGGAAGCACGGAGAGCTGTAGTTTCAGCTGGTGTGGCGTAACCATCTTCTCCC
CAATCTAACACTTTCACTGCAGCAAACCATAACATCCATCAGACGGGAGATCCCCAAAAA
AGACCACCAGTGTCTATCCTAATGAATCATATATATCTTAGTCTAGGAGAAAAGACGGAC
CTGCGACTTCTTGGCCGGCGTAGACACCGCGGTAGACAGTGCCGTAAGTACCGTGAGCGA
GGACGTGCTTCATATCGAGTTTGGAAGGGTCAAT

Last Updated on 06.12.2018 16:11