Sequences from confirmation amplicon


Confirmation sequences for line 253C03 and hit At3g55060 are shown.
Line ID 253C03
Locus At3g55060 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:20403308
Primers used for PCR 16sj - 8474, show primer details
   
Sequence Name 60-K116453-0022-253-C03C-16sj
GenBank Accession LN510349 [GenBank]
Sequencing primer gene specific primer, 16sj: 5'-TCACCACTAAGTTTATAAGCCCATTT-3'
Sequence (A. th genome BLAST matches underlined) 
>60-K116453-0022-253-C03C-16sj
AGCTTTGAGATAGCAATCCAAAAGAAGAAAAGTAAAAATAAAAGAAGAAACAATCATATC
TATAAGCCCCAAAGAATGACCCATCGTCTAAGAAACAATGATAAAGAAAAACCCTTATAC
CAAGAAATCAGGACTTGAGAGGAGATTCAGATGCTTGCTTCCGATTGTGTCTTGTAGTAT
TGTGATCTCTCCTTCTTTCTCTAATACCTTCTCCTCCAATTTCTCTACCATTCCTTTCAA
TGTCTCGTTCTCTGAGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGA
AAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCG
   
Sequence Name 27-K116452-0022-253-C03C-8409
GenBank Accession LN498552 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>27-K116452-0022-253-C03C-8409
GGAATGGTAGAGAAATTGGAGGAGAAGGTATTAGAGAAAGAAGGAGAGATCACAATACTA
CAAGACACAATCGGAAGCAAGCATCTGAATCTCCTCTCAAGTCCTGATTTCTTGGTATAA
GGGTTTTTCTTTATCATTGTTTCTTAGACGATGGGTCATTCTTTGGGGCTTATAGATATG
ATTGTTTCTTCTTTTATTTTTACTTTTCTTCTTTTGGATTGCTTATCTCAAAGCTGGATT
CTCTCTAGTTTCAGATTTGTACTTAAAAATGGGCTTATAACTTTAGTGGTGAA

Last Updated on 06.12.2018 16:11