Sequences from confirmation amplicon


Confirmation sequences for line 253C09 and hit At5g60780 are shown.
Line ID 253C09
Locus At5g60780 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:24452477
Primers used for PCR GL07 - 8474, show primer details
   
Sequence Name 27-K019175w-022-253-C09C-GL07
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer gene specific primer, GL07: 5'-TATAGGTTTTCTGGTTTGCTGTGA-3'
Sequence (A. th genome BLAST matches underlined) 
>27-K019175w-022-253-C09C-GL0
TACAGAACATGGATTTTATTCGTTCTTTATGGATCTTCCATGGGAATTGAATTAACTATC
AACAACGTTATCTCCGGATATTTTTACGACAGGTTCGATTTCTTATTCGTTAAAACCCAA
CTGATGAACTTACTTACTTCTGTCACAAGGTAATAACCTTAATTTGTTTTCTTGTTCTCT
ACGTATTTATCAGGTTTAACCTTAAGCTTCAAACAGCTGGTATAGTAGCAGCCAGCTTTG
GAATGGCTAACTTCATCGCCCGTCCCTTCGGTGGTTACGCTTCTGATGTAGCGGCTCGGG
TTTTTGGCATGAGAGGCCGGTTATGGACCTTATGGATCTTTCAAACCGTAGGAGCTCTTT
TCTGTATCTGGCTAGGTCGAGCTAGTTCACTTCCCATAGCAATCCTAGCAATGATGCTCT
TCTCAATCGGTACACAAGCAGCTTGCGGAGCCCTCTTCGGAGTTGCACCTTTTGTCTCGC
GCCGCTCTCTAGGGCTCATATCGGGACTAACCGGCGCAGGAGGAAACTTCGGGTCCGGTT
TGACTCAACTGCTTTTCTTCTCATCAGCGAGGTTTAGTACAGCTGAGTCCCTCATACTCA
TATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGAT
GGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGA
TGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGA
   
Sequence Name 23-K019175w-022-253-C09C-8409
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>23-K019175w-022-253-C09C-840
AGATTTCCCGGACATGAAGCCATTTACAATTGAATATGAGTATGAGGGACTCAGCTGTAC
TAAACCTCGCTGATGAGAAGAAAAGCAGTTGAGTCAAACCGGACCCGAAGTTTCCTCCTG
CGCCGGTTAGTCCCGATATGAGCCCTAGAGAGCGGCGCGAGACAAAAGGTGCAACTCCGA
AGAGGGCTCCGCAAGCTGCTTGTGTACCGATTGAGAAGAGCATCATTGCTAGGATTGCTA
TGGGAAGTGAACTAGCTCGACCTAGCCAGATACAGAAAAGAGCTCCTACGGTTTGAAAGA
TCCATAAGGTCCATAACCGGCCTCTCATGCCAAAAACCCGAGCCGCTACATCAGAAGCGT
AACCACCGAAGGGACGGGCGATGAAGTTAGCCATTCCAAAGCTGGCTGCTACTATACCAG
CTGTTTGAAGCTTAAGGTTAAACCTGATAAATACGTAGAGAACAAGAAAACAAATTAAGG
TTATTACCTTGTGACAGAAGTAAGTAAGTTCATCAGTTGGGTTTTAACGAATAAGAAATC
GAACCTGTCGTAAAAATATCCGGAGATAACGTTGTTGATAGTTAATTCAATTCCCATGGA
AGATCCATAAAGAACGAATAAAATCCATGTTCTGTAGTTCTTCACAGCAA

Last Updated on 06.12.2018 16:11