Sequences from confirmation amplicon


Confirmation sequences for line 254H12 and hit At4g23700 are shown.
Line ID 254H12
Locus At4g23700 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:12342621
Primers used for PCR RU49 - 8474, show primer details
   
Sequence Name 09-K024450-022-254-H12C-8409
GenBank Accession LN490009 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>09-K024450-022-254-H12C-8409
GTAGATTTCCCGGACATGAAGCCATTTACAATTGAATATATCCTGATGCCACCAACACTG
ATGCCACCGTCGAAACACTTTCGCTTTCCGTTAACAAATTCCCGATCGGTCCAAGCTCAG
GCGTGTCGCTTCTTACGTTTATCCCTGACGCTACAGAACCTTCCGGTGACTTCCCCACCA
AGAAAAGGTTGCTTTTAGAATACTCTTTAATAACTTCGATAACTTCCTCGTAACATTTCA
CGATTTTCTCTTCGTAAATGATATGAGATTCAGAATCCGAATTAGAACGAGAACTTTCTT
TTTCTTTAATCTTTGCTTTAAGTTCGGTTATAGCTTCAATATCAATCAATCTAGTTGCCC
CTGAACAGAGCTGATCTT
   
Sequence Name 10-K024450-022-254-H12C-RU49
GenBank Accession LN490482 [GenBank]
Sequencing primer gene specific primer, RU49: 5'-GAGATAACCGAAGATCAGCTCTGT-3'
Sequence (A. th genome BLAST matches underlined) 
>10-K024450-022-254-H12C-RU49
GGGCAACTAGATTGATTGATATTGCAGCTATAACCGAACTTAAAGCGAAGATTAAAGAAA
AAGAAAGTTCTCGTTCTAATTCGGATTCTGAATCTCATATCATTTACGAAGAGAAAATCG
TGAAATGTTACGAGGAAGTTATCGAAGTTATTAAAGAGTATTCTAAAAGCAACCTTTTCT
TGGTGGGGAAGTCACCGGAAGGTTCTGTAGCGTCAGGGATAAACGTAAGAAGCGACACGC
CTGAGCTTGGACCGATCGGGAATTTGTTAACGGAAAGCGAAAGTGTTTCGACGGTGGCAT
CAGTGTTGGTGGCATCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTAC
ATGGATCAGCAATGAGTATGATGGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATT
TTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTG
ATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCT
AATTCGGAAATCTTTATTTCGACGTGTCTACATTCA
Primers used for PCR 05bd - 8474, show primer details
   
Sequence Name 59-K118893-0022-254-H12C-05bd
GenBank Accession LN510116 [GenBank]
Sequencing primer gene specific primer, 05bd: 5'-AACACATAAATCATCACAACGCAT-3'
Sequence (A. th genome BLAST matches underlined) 
>59-K118893-0022-254-H12C-05bd
CACATTTAGCCAGCATCGTTTTTTTTTTTAAAGTCTCCCAAAATAGTCATTAACTACAAA
ACATTCGATAAAGTCGCCAATTAAATTCCAGGGAAGTTCGGTAATTTTGTAAACCAAATT
AATTTCTATTTTGAAAACTAATTTGAAAACAAAACAAAAAACTGTGAAAAAACTATCCTA
TATTATTCCCCGTGGGTAGTTATTATATAAAATCAAGATGTATAACATTTTATTTTATTC
CCCGAAGGTAGTTATTTTTTTGTCAAACCGTTGGTAGTTCAATTGATGTATATAATTAGT
GGGCCATGTAAATTAAAAAAAAAAAATGTCCCGGAAACCTTACCATATAGAAATCATTTA
TTCCCAATTATTATTTACAATATGAAACACTTGTTATTATACAAGCCAATCTTTTGACCT
TTCAAGTCTGGGAATCCCAAATAAAAAATTCACTAAGGACTCTCAAAATCCTCAACCAGC
GACTCTTCAGTCGGGACGTTCTTCAAAATTCCAACCGGACGGCTGGCTATATACGGTTGC
TATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACTTGGATCACCAATGAGTATG
ATGGTCAATATGGAGAAAAAAAAAGAGTAATTCCCAATTTTTTTTCAATTCAAAAATGTA
AATTTCCGCA
   
Sequence Name 19-K118892-0022-254-H12C-8409
GenBank Accession LN494979 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>19-K118892-0022-254-H12C-8409
CATTTCATTGAATTAGCAACAGTATATAGCAAGCCGTCCGGTCGGAATTTCGAAGAACGT
CACGACTGAAGAGTCGCTGGTTGAGGATTCTGAGAGTCCTTAGTGAATTTTCTATTTGGG
ATTCCAAGACTCGAAAGGTCAAAAGATTGGCTTGTATAATAACATGTGTTTCATATTGTA
AATAATAATTGCGAATAAATGATTTCTATATGCTAAGGTCTCCGTGACATTTTCTTCTTT
TAATTTACATGACCAACTAATTATATACATCAATTGAACTACCAACGGTTTGACAAAAAA
ATAACTACCTACGGTGAATAAAATAAAATGTTATACATCTTGATTTTATATAATAACTAC
CAACGGTGAATAATATAGTATAGTTTTTTCACAGTTTTCTGTTTTGTCTTCAAATTAGTT
TTCAAAATAGAAATTAATTTGGTTTACAAAATTACCGAACTTCCATGGAATTTAATTGAC
GACTTTATCGAATGTTTTGTAGTTAATGACTATTTTGGGAGACTTCTAAAAAAAAAAACA
ATGCTGGCTTAAATTTGTTTGTTTATGCATATATGCGTTGT

Last Updated on 06.12.2018 16:11