Genotyping and primer details


Genotyping and primer details for 254H12 - At4g23700
Line ID 254H12
Locus At4g23700 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:12342621 of line 254H12 Chr4:12342621
   
Insertion allele amplicon 8474 - 05bd
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 05bd: 5'-AACACATAAATCATCACAACGCAT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:12342041
   
Insertion allele amplicon 8474 - RU49
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer RU49: 5'-GAGATAACCGAAGATCAGCTCTGT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:12342962
   
wt amplicon (over InsPos) 05bd - RU49
wt amplicon start Chr4:12342041
wt amplicon end Chr4:12342962
wt amplicon size 922 bp
Locus specific primer RU49: 5'-GAGATAACCGAAGATCAGCTCTGT-3'
2nd locus specific primer 05bd: 5'-AACACATAAATCATCACAACGCAT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11