Sequences from confirmation amplicon


Confirmation sequences for line 255C11 and hit At3g05560 are shown.
Line ID 255C11
Locus At3g05560 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:1614756
Primers used for PCR 16qi - 8474, show primer details
   
Sequence Name 24-K115435-0022-255-C11C-16qi
GenBank Accession LN497124 [GenBank]
Sequencing primer gene specific primer, 16qi: 5'-TTTTTAGCTTCATTTCCTCTTGGA-3'
Sequence (A. th genome BLAST matches underlined) 
>24-K115435-0022-255-C11C-16qi
CACTTCCCATTTTTCAGGTTTTGAGTTAGTCATAGTAGCTAGAAAACGGCTTATAAAGTA
TATAGAGAATATAGGCTTCTTTATTTGTATCAGTATGATTTGTCAATTGATCTATAGACA
CAGTCTTATGGATTGTTTTTTGGATTTTACAGAGAAGTCTTGGGAAAATGAGTCGTGGAG
GTGCAGCTGTTGCCAAGGGAAAGAAGAAGGGAGTTTCCTTCACCATTGATTGTTCAAAGC
CTGTCGATGACAAGATCATGGAGATTGCTTCCCTTGAGAAGTTTCTTCAGGATATATTCA
ATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAA
TATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAG
   
Sequence Name 08-K115434-0022-255-C11C-8409
GenBank Accession LN489862 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>08-K115434-0022-255-C11C-8409
AACTTCTCAAGGGAAGCAATCTCCATGATCTTGTCATCGACAGGCTTTGAACAATCAATG
GTGAAGGAAACTCCCTTCTTCTTTCCCTTGGCAACAGCTGCACCTCCACGACTCATTTTC
CCAAGACTTCTCTGTAAAATCCAAAAAACAATCCATAAGACTGTGTCTATAGATCATTGA
CAAATCATACTGATACAAATAAAGAAGCCTATATTCTCTATATACTTTATAAGCCATTTT
CTAGCTACTATGACTAACTCAAAACCTGGAAAATGGGAAGTGAGATCTGTATCGCAGATT
CGACTTTCAATCTAACATAGTCAATTACACTCTAAGTAGATGAAAAATGAAGCTAATCCA
AGAGG

Last Updated on 06.12.2018 16:11