Sequences from confirmation amplicon


Confirmation sequences for line 255G10 and hit At3g18180 are shown.
Line ID 255G10
Locus At3g18180 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:6230789
Primers used for PCR 08xu - 8474, show primer details
   
Sequence Name 25-K119478-0022-255-G10C-08xu
GenBank Accession LN497687 [GenBank]
Sequencing primer gene specific primer, 08xu: 5'-GTTATTGTAGAGAGCCTAGAGGCAG-3'
Sequence (A. th genome BLAST matches underlined) 
>25-K119478-0022-255-G10C-08xu
TTTCGCATCATATACTGGTAAATTCTTGTAGTTGGAATGTTGGATCGTAATATTGTTATC
ATAAAACAATTATATTTATCGAAATTTCTGAACTGTCAGTTATTATTCTGATGAGGCTCA
ATCGACTAACCAGAGTAAACCGATATCGATTAGAAAAAGAATTATGTGGCTTACACTAAA
TTTTATGTAACTTTTTACAGTTTTTTGGTCAGCTTTAATCTGTTTTGAATAGCTTTTCCC
CCATTTAACGGATATTTTTGTCATGCTTTCTTTCCTTCTCTTATTTTGGTTGCAAATTAA
ATTGCATGTTTTAATGATTTCTGTAATTTTGATATGGAGCTAAATGACTCATCACTAACT
CAATATGAAGCAAATAAAGAAAAAACTCCCAAGACTTCTCCTTCTTCCTTAAACTCTACC
AATATAAGATCGAACCACTTTGAACATGCTCTAGCTAGTTCTCTTCTTTAAACCAGTACC
CTTTATATTCATTCTCAATAACTAAAACGTTATAGTGAAAAAAAAAACACAGATAATATC
GTCAAACAAATTCTTAAATACCTCACATAATCCTCTCGCTCTCCCACCTAGCTGGACCTT
CATATCTCTACATTAGCCAGTAAACTCTGATTATAGAGTTTCTTGCTGAAGAAGAAAGAA
AAGTTTTGTGTAACTTAAGAGTCAAGAGATGACAAAGAAGGATATTCTTTACGATACAGT
ACTTGCTCGTAGTTTTAGCAAAACTGATCAGAAGAGACTTTGCTGTGGAGCTTTCATTGC
CTCTCTTCTCTTGGTCCTCACTCTTTGCACTGTCGTTAAACCTTATCTCAGCCCTTTACC
AATCGGTAAGTCTCTTACCACGTTTTGCCGAAAAGCAAGATAACGCAAAAACAATCATTT
TAACGGGTATCTCTTTCTTT
   
Sequence Name 01-K119477-0022-255-G10C-8409
GenBank Accession LN486806 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>01-K119477-0022-255-G10C-8409
GCTTACATCGCAAACTCTTTGCTTCCTAGTGTGTTACAGTGACAGATAGGTTTCTCCATT
TTATTGCACTCTGAAGATTCAGTCAGGACAAAATCAAAAGAACAAACTGTATCATACTAT
ACAGAAAATGACTAAAAACCATAACAAGACAATTGTAGAGAAAATTCTGAAGTATATTGA
CCTGAAATAACTTCTTCTTTGGAGATTGTAGTGTTGGTAGTTAATTCTGTTATACTCAGC
ATCCTTAGACCAGTGCCAACTGATAACTGTAACTCCACTGAAGAAAGAAAGAAAGAGATA
CACGTTAAAATGATTGTTTTTGCGTTATCTGGCTTTTCGGCAAAACGTGGTAAGAGACTT
ACCGATTGGTAAAGGGCTGAGATAAGGTTTAACGACAGTGCAAAGAGTGAGGACCAAGAG
AAGAGAGGCAATGAAAGCTCCACAGCAAAGTCTCTTCTGATCAGTTTTGCTAAAACTACG
AGCAAGTACTGTATCGTAAAGAATATCCTTCTTTGTCATCTCTTGACTCTTAAGTTACAC
AAAACTTTTCTTTCTTCTTCAGCAAGAAACTCTATAATCAGAGTTTACTGGCTAATGTAG
AGATATGAAGGTCAAGCTAGGTGGGAGAGCGAGAGGATTATGTGAGGTATTTAAGAATTT
GTTTGACGATATTATCTGTGTTTTTTTTTTCACTATAACGTTTTAGTTATTGAGAATGAA
TATAAAGGGTACTGGTTTAAAGAAAAAAACTAGCTAGAGCATGTTCAAAGTGGTT

Last Updated on 06.12.2018 16:11