Sequences from confirmation amplicon


Confirmation sequences for line 256A08 and hit At3g54040 are shown.
Line ID 256A08
Locus At3g54040 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:20014246
Primers used for PCR 19gw - 8474, show primer details
   
Sequence Name 24-K111953-0022-256-A08C-19gw
GenBank Accession LN497053 [GenBank]
Sequencing primer gene specific primer, 19gw: 5'-GGACTATTGCCCTAACATTCTTGA-3'
Sequence (A. th genome BLAST matches underlined) 
>24-K111953-0022-256-A08C-19gw
CATACATAATAACTCATGCATACATGGCCAAACTTGTCAAATGATAGAGATTATTTGTTG
AAAAGAAATTTTGGTAGAAGATTTAATTATAGTTTATTTGCCACAAGTTTTGTTACGAAA
TTCATTCTTATTTTCTAATAAGTGAAAATAATGTAGTACCAAAATAAGAACGTTTTTCAA
GGAATGGCCGTAACTAGAAAAGTAAAAAGTGTGTAAATTATGATCTTTATCATGGCTTAT
TAATGAAGTAAGAATCGAATATTTGTCTGTTTTCGTAGATTCCTCGTGTTTCTTACACAC
GAAACTCACTTTCATGTTTTTTTCGGTATTTCGTATCTTCAAAGCAAGGAATTTCGGTAG
GAGCTATAGATGGTTATGTTTCACTTAACACAATTATTTTTTTAATGTAATAGTGTACGT
TGGTCGTAAATAAATAAATAATAGTGTACATAGTGTACAGGATATATTCAATTGTAAATG
GCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAA
AAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCA
   
Sequence Name 08-K111952-0022-256-A08C-8409
GenBank Accession LN489792 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>08-K111952-0022-256-A08C-8409
TCCTGTACACTATGTACACTATTATTTATTTATTTACGACCAACGTACACTATTACATTA
AAAAAATAATTGTGTTAAGTGAAACATAACCATCTATAGCTCCTACCGAAATTCCTTGCT
TTGAAGATACGAAATACCGAAAAAAACATGAAAGTGAGTTTCGTGTGTAAGAAACACGAG
GAATCTACGAAAACAGACAAATATTCGATTCTTACTTCATTAATAAGCCATGATAAAGAT
CATAATTTACACACTTTTTACTTTTCTAGTTACGGCCATTCCTTGAAAAACGTTCTTATT
TTGGTACTACATTATTTTCACTTATTAGAAAATAAGAATGAATTTCGTAACAAAACTTGT
GGCAAATAAACTATAATTAAATCTTCTACCAAAATTTCTTTTCAACAAATAATCTCTATC
ATTTGACAAGTTTGGCCATGTATGCATGAGTTATTATGTATGGTACCTTCACCGGCGGCG
AGGTTGAAATAGAGATCAAGAATGTTAGGGCAATAGTC

Last Updated on 06.12.2018 16:11