Sequences from confirmation amplicon


Confirmation sequences for line 256B07 and hit At1g80480 are shown.
Line ID 256B07
Locus At1g80480 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:30259767
Primers used for PCR CS22 - 8474, show primer details
   
Sequence Name 66-K031320-022-256-B07C-8409
GenBank Accession LN511522 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>66-K031320-022-256-B07C-8409
CATTTACATTGAATATATCCTGATAGTCATTACTCATTCCCCAGATTCTCTAAGCATACT
AAATGAAAATGCCCCCTTTCCCAACCTGTAGTCTCTATGACTATATGATCGAACTTTCCT
TTCTTGGTATTGACTAATTCTCCAATCATCCTGACAAGATCACCCCTAACAGTGCAACAA
AGGCAGCCATTGTTGAGCATCACAATATCCTCAGCTCCAATAGATTTAGAAGCAACAAGT
GACCCATCAATGTCAACTTCACCAAACTGAGAGAATTCACATGAAACATGAATTAGAAAG
CAACACCATTATAACTGAATTCAGGCGAGGATAATAAGCTAACCTCATTCTCAATCACGG
CTATTCGCTTCCCATGATCTCTGGTCAGTATATGATTCAGCAATGTTGTCTGTGGAAAAA
CAAAACATTGCAGTATAATTAGTCCAACATTTCTTTTACTTAGCAGGGCTAATTTTAAAC
CCTAGAATAGATATGAAATGATGAGAAACACTGACTGACCTTGCCAGAGCCCAAAAAACC
GGTGATAATAGTAGCCGGAATCCGGTTATCTGGCGGAATCTTAGTGGTGACATCAGAATC
T
   
Sequence Name 67-K031320-022-256-B07C-CS22
GenBank Accession LN511731 [GenBank]
Sequencing primer gene specific primer, CS22: 5'-CTACGCAGACTGAAGATTCTGATG-3'
Sequence (A. th genome BLAST matches underlined) 
>67-K031320-022-256-B07C-CS22
ACCGGATTCCGGCTCTATTATCACCGGTTTTTTGGGCTCTGGCAAGGTCAGTCAGTGTTT
CTCATCATTTCATATCTATTCTAGGGTTTAAAATTAGCCCTGCTAAGTAAAAGAAATGTT
GGACTAATTATACTGCAATGTTTTGTTTTTCCACAGACAACATTGCTGAATCATATACTG
ACCAGAGATCATGGGAAGCGAATAGCCGTGATTGAGAATGAGGTTAGCTTATTATCCTCG
CCTGAATTCAGTTATAATGGTGTTGCTTTCTAATTCATGTTTCATGTGAATTCTCTCAGT
TTGGTGAAGTTGACATTGATGGGTCACTTGTTGCTTCTAAATCTATTGGAGCTGAGGATA
TTGTGATGCTCAACAATGGCTGCCTTTGTTGCACTGTTAGGGGTGATCTTGTCAGGATGA
TTGGAGAATTAGTCAATACCAAGAAAGGAAAGTTCGATCATATAGTCATAGAGACTACAG
GTTGGGAAAGGGGGCATTTTCATTTAGTATGCTTAGAGAATCTGGGGAATGAGTAATGAC
TATCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAA
TGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCA
AAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAA
TTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCT
TTATNTCGACGTGTCTACT

Last Updated on 06.12.2018 16:11