Genotyping and primer details


Genotyping and primer details for 256B07 - At1g80480
Line ID 256B07
Locus At1g80480 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:30259767 of line 256B07 Chr1:30259767
   
Insertion allele amplicon 8474 - CS22
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer CS22: 5'-CTACGCAGACTGAAGATTCTGATG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:30260359
   
wt amplicon (over InsPos) 14ee - CS22
wt amplicon start Chr1:30259311
wt amplicon end Chr1:30260359
wt amplicon size 1049 bp
Locus specific primer CS22: 5'-CTACGCAGACTGAAGATTCTGATG-3'
2nd locus specific primer 14ee: 5'-TTGGATATGCCAACAAAAACAATA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11