Sequences from confirmation amplicon


Confirmation sequences for line 256F07 and hit At2g39030 are shown.
Line ID 256F07
Locus At2g39030 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:16298656
Primers used for PCR GG48 - 8474, show primer details
   
Sequence Name 91-K100901-0022-256-F07C-8409
GenBank Accession LN515098 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>91-K100901-0022-256-F07C-8409
CATTTACAATTGAACTACATGTTCTGTTCTTCCCGAACTACCCAAGTTTTCTAGCGAAAC
AGGGTTTCTACATCGAGGATATCTTCATGAGAGAACCTTACAGACGAAAAGGCTTCGGAA
AACTGCTACTGACCGCTGTGGCAAAGCAAGCAGTGAAGTTGGGTGTCGGAAGAGTGGAGT
GGATTGTGATCGATTGGAATGTGAATGCTATCAACTTCTATGAGCAGATGGGTGCGCAGG
TTTTTAAGGAATGGAGACTTTGTAGGCTCACTGGCGATGCACTTCAAGCTATTGACAAGC
TAAACATCTAGCAGCAT
   
Sequence Name 91-K100907-0022-256-F07C-GG48
GenBank Accession LN515099 [GenBank]
Sequencing primer gene specific primer, GG48: 5'-CATGCTGCTAGATGTTTAGCTTGT-3'
Sequence (A. th genome BLAST matches underlined) 
>91-K100907-0022-256-F07C-GG48
AAGTCTCCATTCCTTAAAACCTGCGCACCCATCTGCTCATAGAAGTTGATAGCATTCACA
TTCCAATCGATCACAATCCACTCCACTCTTCCGACACCCAACTTCACTGCTTGCTTTGCC
ACAGCGGTCAGTAGCAGTTTTCCGAAGCCTTTTCGTCTGTAAGGTTCTCTCATGAAGATA
TCCTCGATGTAGAAACCCTGTTTCGCTAGAAAACTTGGGTAGTTCGGGAAGAACAGAACA
TGTAGTTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTAT
GATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGT
AGATGTCC

Last Updated on 06.12.2018 16:11