Sequences from confirmation amplicon


Confirmation sequences for line 256F10 and hit At3g47750 are shown.
Line ID 256F10
Locus At3g47750 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:17607484
Primers used for PCR 13vh - 8474, show primer details
   
Sequence Name 50-K117914-0022-256-F10C-13vh
GenBank Accession LN507306 [GenBank]
Sequencing primer gene specific primer, 13vh: 5'-AGGTACAAAACAATGTGCACTCAG-3'
Sequence (A. th genome BLAST matches underlined) 
>50-K117914-0022-256-F10C-13vh
CGGACTACTTTACACGCCTATCTTGTACTTTGAAATTACCCTTGTAAGAAGAGTTATACC
AGATGGTCACATTGAACTTGTTCCTATCAGTGTCCAAGAGATCGTATGCTAATATAAAAC
CAGAGAAAGAGTCAGAAGTTGCAAAAGTAAAAAAATAGAGACATATGATAAGAGAAGTGT
AATAACCTGCAGCGACCTCATTGATGATCTCTTCATGATTTCCTTGACGATAGCCCTTAA
AGATCTCATCATTGACCTCTACTGAGTTATTTCTCCAGAGATTCAATCCTTGAACGCATC
TCACCTCTGGTTTGACAAATTAACTAAGTTAATCAGCTCCAAGACTGTTTTCCAGAAGCT
AGCTTTGAGTGATGCCCAAGAAATATTATTTTTAATAAAACTTGCAACATGGAATGCACT
CAGATTGCATTTAAACTTAAACAGAAAATTTGCTCAAGTAAAGTCAACATATCTTAATTA
TGTTCAATTGGACAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATG
GATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTT
TTCAATTCAAAAATGTAGATGTCCGC
   
Sequence Name 10-K117913-0022-256-F10C-8409
GenBank Accession LN490852 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>10-K117913-0022-256-F10C-8409
CTTTACATTGAATATATCCTGTCCAATTGAACATAATTAAGATATGTTGACTTTACTTGA
GCAAATTTTCTGTTTAAGTTTAAATGCAATCTGAGTGCATTCCATGTTGCAAGTTTTATT
AAAAATAATATTTCTTGGGCATCACTCAAAGCTAGCTTCTGGAAAACAGTCTTGGAGCTG
ATTAACTTAGTTAATTTGTCAAACCAGAGGTGAGATGCGTTCAAGGATTGAATCTCTGGA
GAAATAACTCAGTAGAGGTCAATGATGAGATCTTTAAGGGCTATCGTCAAGGAAATCATG
AAGAGATCATCAATGAGGTCGCTGCAGGTTATTACACTTCTCTTATCATATGTCTCTATT
TTTTTACTTTTGCAACTTCAGACTCTTTCTCTGGTTTTATATTAGCATACGATCTCTTGG
ACACTGATAGGAACAAGTTCAATGTGACCATCTGGTATAACTCTTCTTACAAGGGTAATT
TCAAAGTACAAGATAGGCGTGTAAAGTATGTCCGAGTACCTCGCTCAATCAATATGGTAA
GAAACTGAGTGCACATTGT

Last Updated on 06.12.2018 16:11