Sequences from confirmation amplicon


Confirmation sequences for line 256H07 and hit At1g55520 are shown.
Line ID 256H07
Locus At1g55520 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:20727365
Primers used for PCR 55fr - 8474, show primer details
   
Sequence Name 43-K111327-0022-256-H07C-55fr
GenBank Accession LN504877 [GenBank]
Sequencing primer gene specific primer, 55fr: 5'-AAAAGCCTTCTCCTTTTTCAAATC-3'
Sequence (A. th genome BLAST matches underlined) 
>43-K111327-0022-256-H07C-55fr
GGGATTTCTTCCATCTGTTGGGTTACGAAACCCTAGATTTCGAAATTCCGGATTGCTAAC
TAGCGAAAGGTCTGATTTTGTTTCGTACCCTTTGTTCCTAATGGATTCAATTCCACGGAT
TTTATCTGAATTGAGTTTCTTCGTAGTTGCTTGTTACGAATTGGATCTCATGTTCTGAAG
TTGTGTGTTTCATAGCTTTTCGTTGTTTGATTGTGAAACAATGTTTCTAGATTTCAATTA
TCAAAACTGGTTTTTAGATAACCAGATTTGGTCAATGAAATTGTAAAGCTTGAAAACAAT
TTTGTTTCGTGGATGCTTGTTGGTTTGGATCGACATGAGTTTCTGTTTTGCCCTATTTAA
GACGAGATTTATAGCTGATGTGTGTGTGTTTACGTTGATTCTTATGTTAGAGAATGGCTG
ATCAAGGAACGGAAGGGAGCCAGCCAGTTGACCTTACTAAACATCCTTCAGGGATTGTTC
CTACTCTTCAGTGAGTTCTCTCTTTTGTGTCTCTGTTTTAAGATTCTGAGTTTGTAAATG
GCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAA
AAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGA
   
Sequence Name 35-K111326-0022-256-H07C-8409
GenBank Accession LN501875 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>35-K111326-0022-256-H07C-8409
CATTACAACTCAGAATCTTAAAACAGAGACACAAAAGAGAGAACTCACTGAAGAGTAGGA
ACAATCCCTGAAGGATGTTTAGTAAGGTCAACTGGCTGGCTCCCTTCCGTTCCTTGATCA
GCCATTCTCTAACATAAGAATCAACGTAAACACACACACATCAGCTATAAATCTCGTCTT
AAATAGGGCAAAACAGAAACTCATGTCGATCCAAACCAACAAGCATCCACGAAACAAAAT
TGTTTTCAAGCTTTACAATTTCATTGACCAAATCTGGTTATCTAAAAACCAGTTTTGATA
ATTGAAATCTAGAAACATTGTTTCACAATCAAACAACGAAAAGCTATGAAACACACAACT
TCAGAACATGAGATCCAATTCGTAACAAGCAACTACGAAGAAACTCAATTCAGATAAAAT
CCGTGGAATTGAATCCATTAGGAACAAAGGGTACGAAACAAAATCAGACCTTTCGCTAGT
TAGCAATCCGGAATTTCGAAATCTAGGGTTTCGTAAACCCAACAGATTGGAAGAAATTCC
CCCGAAAAGGCGAAAGGAAGGTGAATGATTTGAAAAAGA

Last Updated on 06.12.2018 16:11