Sequences from confirmation amplicon


Confirmation sequences for line 256H07 and hit At3g07390 are shown.
Line ID 256H07
Locus At3g07390 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:2365402
Primers used for PCR BD80 - 8474, show primer details
   
Sequence Name 08-K028341-022-256-H07C-8409
GenBank Accession LN489594 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>08-K028341-022-256-H07C-8409
CATTTACAATTGAATCAATCTCCATAACTTCAGGGGCATTGCCCTTCTATATAAACATCA
CATGTCCCTGTGTCTTAAAANTACCTCTTATCAAACACCAAACCACACCAGAACAGAACT
CAGCCATGGCTTCTTCTTCTTCTTCTCTTCTAATCCTAGCCGTTGCTTGTTTCGTCTCGC
TAATCTCACCGGCGATTTCACAACAGGCTTGCAAATCACAGAACTTGAACTCCGCCGGTC
CGTTCGATAGTTGCGAAGACCTTCCGGTACTCAATTCCTACCTCCATTACACCTACAATT
CCTCAAATTCATCTCTCTCCGTCGCTTTCGTCGCCACTCCATCTCAAGCCAACGGTGGCT
GGGTCGCTTGGGCTATTAACCCTACGGGGACTA
   
Sequence Name 08-K028341-022-256-H07C-BD80
GenBank Accession LN489595 [GenBank]
Sequencing primer gene specific primer, BD80: 5'-CTTAGTCCCCGTAGGGTTAATAGC-3'
Sequence (A. th genome BLAST matches underlined) 
>08-K028341-022-256-H07C-BD80
TGGCTTGAATGGAGTGGCGACGAAAGCGACGGAGAGAGATGAATTTGAGGAATTGTAGGT
GTAATGGAGGTAGGAATTGAGTACCGGAAGGTCTTCGCAACTATCGAACGGACCGGCGGA
GTTCAAGTTCTGTGATTTGCAAGCCTGTTGTGAAATCGCCGGTGAGATTAGCGAGACGAA
ACAAGCAACGGCTAGGATTAGAAGAGAAGAAGAAGAAGAAGCCATGGCTGAGTTCTGTTC
TGGTGTGGTTTGGTGTTTGATAAGAGGTATTTTAAGACACAGGGACATGTGATGTTTATA
TAGAAGGGCAATGCCCTGAAGTTATGGAGATTGATTCAATTGTAAATGGCTTCATGTCCG
GGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAA
TTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAG
TACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACA
AATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTACAT

Last Updated on 06.12.2018 16:11