Sequences from confirmation amplicon


Confirmation sequences for line 257G10 and hit At5g03290 are shown.
Line ID 257G10
Locus At5g03290 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:795694
Primers used for PCR GC72 - 8474, show primer details
   
Sequence Name 27-K100995-0022-257-G10C-8409
GenBank Accession LN498306 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>27-K100995-0022-257-G10C-8409
TGAGCCATTTACATTGATCTTTCAACATTGGATGCATAAATGATACTCTTTAGCCCGTAA
ATGATGAAATTGGAATGATATATTGTTTGAAGGACCAACCTTTCCAGCGATATCAGGTGC
AGAACCGTGAACTGCTTCAGCAAGGGCTACACCATCTTCTCCAATATTACAACTGCGTCA
AAGATAATAAGGCCTTAAGATGATGTAGCTCATAATCATACTAAAGATCACTATTACACA
AGATGAAGGAACAAGTGTTTTTACCTTGGAGTCAATCCTAGTCCTCCAACCAATCCGGCA
CACAGATCGCTAATAATGTCACCGTACAGGTTTGGCATCACCAAGACATCAAAAAGTGCT
GGGTTTTTCACAAGCTGCAGGTATCTCAAGATTATCAGTCGCATATTCCACATGTAAAAA
CAGTGGAAAAGCAGAACTCAAATACAAGTTGCTAAGAAACCAAACTTCTAAATCTACAAG
GTGATCCCGAGTCCCGACCACTAACTTAATTAACGAAAAAATAGTAGATTCGGTGTCATA
GCTNACCATCATACAGCAGTTGTCAATAACGACCTC
   
Sequence Name 27-K100999-0022-257-G10C-GC72
GenBank Accession LN498307 [GenBank]
Sequencing primer gene specific primer, GC72: 5'-AGATCACTTACGAGGAGGTCGTTA-3'
Sequence (A. th genome BLAST matches underlined) 
>27-K100999-0022-257-G10C-GC72
AGGTAGCTTGACCCGAATCTCTATTTTTTCGTTATTAAGTTAGTGGTCGGGACTCGGGAT
CACCTTGTAGATTTAGAAGTTTGGTTTCTTAGCAACTTGTATTTGAGTTCTGCTTTTCCA
CTGTTTTTACATGTGGAATATGCGACTGATAATCTTGAGATACCTGCAGCTTGTGAAAAA
CCCAGCACTTTTTGATGTCTTGGTGATGCCAAACCTGTACGGTGACATTATTAGCGATCT
GTGTGCCGGATTGGTTGGAGGACTAGGATTGACTCCNAGGTAAAAACACTTGTTCCTTCA
TCTTGTGTAATAGTGATCTTTAGTATGATTATGAGCTACATCATCTTAAGGCCTTATTAT
CTTTGACGCANTTGTAATATTGGAGAAGATGGTGTAGCCCTTGCTGAAGCAGTTCACGGT
TCTGCACCTGATATCGCTGGAAAGGTTGGTCCTTCAAACAATATATCATTCCAATTTCAT
CATTTACGGGCTAAAGAGTATCATTTATGCATCCNATGTTGAAAGATCAATTGTAAATGG
CTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAA
AGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCC

Last Updated on 06.12.2018 16:11