Sequences from confirmation amplicon


Confirmation sequences for line 259A08 and hit At1g42470 are shown.
Line ID 259A08
Locus At1g42470 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:15930817
Primers used for PCR MM60 - 8474, show primer details
   
Sequence Name 39-K020244-022-259-A08C-8409
GenBank Accession LN503201 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>39-K020244-022-259-A08C-8409
TAGATTTCCCGGACATGAAGCCATTTACAATTGAATATATCCTGACATTTACAGCTGGCA
TTTTAATGAAAGCTAAAATCTCGGCTAAACTTGCTAGAGTAATCGATGGTCCAACTTCCA
TAAGGGCATTGCTAATTCTTCTTTCCAAAGGCAGCTCTTGCTCTTGCCTCTTGACAGCAT
GAACCAGGATGCACATATTATCAACACCGACCTGTCGATAAAAATATTCCAAGTAGAAGT
AAAAAAAAATTAAGAACAATATGAAGAACGTCGCCACAACATCAGCTAACATATTGAAGT
TGCAAGCAATATTAGAAATAGACACGACTTACAGCCAAAACAAGAAAAGGTATAACTTCC
ATTATGATTAGAGTCGATTTCATCCCAACGGCACTGAAAAATCCTACAGAGCCAAGGACA
GAAAGCATGACAAGTAGAACACCAGATAGACCAAGCAGAACCT
   
Sequence Name 40-K020244-022-259-A08C-MM60
GenBank Accession LN503557 [GenBank]
Sequencing primer gene specific primer, MM60: 5'-TCTTAATTTCAGGTTCTGCTTGGT-3'
Sequence (A. th genome BLAST matches underlined) 
>40-K020244-022-259-A08C-MM60
TCTGGTGTTCTACTTGTCATGCTTTCTGTCCTTGGCTCTGTAGGATTTTTCAGTGCCGTT
GGGATGAAATCGACTCTAATCATAATGGAAGTTATACCTTTTCTTGTTTTGGCTGTAAGT
CGTGTCTATTTCTAATATTGCTTGCAACTTCAATATGTTAGCTGATGTTGTGGCGACGTT
CTTCATATTGTTCTTAATTTTTTTTTACTTCTACTTGGAATATTTTTATCGACAGGTCGG
TGTTGATAATATGTGCATCCTGGTTCATGCTGTCAAGAGGCAAGAGCAAGAGCTGCCTTT
GGAAAGAAGAATTAGCAATGCCCTTATGGAAGTTGGACCATCGATTACTCTAGCAAGTTT
AGCCGAGATTTTAGCTTTCATTAAAATGCCAGCTGTAAATGTCAGGATATATTCAATTGT
AAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGG
AGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCG
TTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTA

Last Updated on 06.12.2018 16:11