Sequences from confirmation amplicon


Confirmation sequences for line 259D11 and hit At2g27315 are shown.
Line ID 259D11
Locus At2g27315 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:11690249
Primers used for PCR KB19 - 8474, show primer details
   
Sequence Name 70-K100816-0022-259-D11C-8409
GenBank Accession LN512334 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>70-K100816-0022-259-D11C-8409
CATTTACATTGAATATATCCAAAAAAATATTATAACTTACTCATCTTCTGTTTCATAATT
TGAACTAGTAAACACAATACATTTACATTAGTCAACTTAACTTAATGTAATAAAAATTAA
TACGGTGGACCTAAACATGAGTTACATTTTGAAACAAAACTTTAGACTGATCTGTTACTT
GTCGATGTATTGATTAAAATCTTGTTTCCTGATCCTATAAATTCTAAAATTTCTTCCATA
TCTAAAACCATCCCAAGAATCTAAGTCTAAAGTCTAAACCAAAGCCAAGACAAACGAAAG
GAAAAAAGAAGAAAGATGGGAAGCAAAGCTATTGTATCAGTCTTGTTAATGGCATCCCTT
TGTGGTACCATTTTTGTTACACAAGGAGTAGCTGAAATGCAAAAGCCACCGATGTTTCCA
GGACTTTTCCAACCAGGCTCACCCGTTGATCTTGTAAAATGTATGTCGTCTCTCTCCAAC
GTTGAAGGATGTGTGCTCGAAATCTCTAAATCAATTTTCTCCGGCAAATTTGAAAATATT
GAAGGCGCATGTTGCAAGGCGTTTTCGGCCTTAGATGCCAACTGTTGGCCCGAAATGTTT
CCGTTGAATCCGTTTTTCCCTCCTCTCCTCAG
   
Sequence Name 70-K100828-0022-259-D11C-KB19
GenBank Accession LN512336 [GenBank]
Sequencing primer gene specific primer, KB19: 5'-CACAAATATCCTTGAGGAGAGGAG-3'
Sequence (A. th genome BLAST matches underlined) 
>70-K100828-0022-259-D11C-KB19
TTCGGGCCACAGTTGGCATCTAAGGCCGAAAACGCCTTGCAACATGCGCCTTCAATATTT
TCAAATTTGCCGGAGAAAATTGATTTAGAGATTTCGAGCACACATCCTTCAACGTTGGAG
AGAGACGACATACATTTTACAAGATCAACGGGTGAGCCTGGTTGGAAAAGTCCTGGAAAC
ATCGGTGGCTTTTGCATTTCAGCTACTCCTTGTGTAACAAAAATGGTACCACAAAGGGAT
GCCATTAACAAGACTGATACAATAGCTTTGCTTCCCATCTTTCTTCTTTTTTCCTTTCGT
TTGTCTTGGCTTTGGTTTAGACTTTAGACTTAGATTCTTGGGATGGTTTTAGATATGGAA
GAAATTTTAGAATTTATAGGATCAGGAAACAAGATTTTAATCAATACATCGACAAGTAAC
AGATCAGTCTAAAGTTTTGTTTCAAAATGTAACTCATGTTTAGGTCCACCGTATTAATTT
TTATTACATTAAGTTAAGTTGACTAATGTAAATGTATTGTGTTTACTAGTTCAAATTATG
AAACAGAAGATGAGTAAGTTATAATATTTTTTTGGGATATATTCAATTGTAAATGGCTTC
ATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAANAAGAA
AGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATG

Last Updated on 06.12.2018 16:11