Sequences from confirmation amplicon


Confirmation sequences for line 259H02 and hit At1g62330 are shown.
Line ID 259H02
Locus At1g62330 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:23049640
Primers used for PCR 34jd - 8474, show primer details
   
Sequence Name 48-K113531-0022-259-H02C-34jd
GenBank Accession LN506555 [GenBank]
Sequencing primer gene specific primer, 34jd: 5'-CTTCGTTCCGAAGATACAAGAAAC-3'
Sequence (A. th genome BLAST matches underlined) 
>48-K113531-0022-259-H02C-34jd
TGGATCACACCTTGCACCAGTTGACCCGTATCTCGTCGGTCCAAAATTTGCGTCATTCAT
ATTGGACAAGAAAGCAGGTCCTCTCCATAAGGCTTCTAAGTATCTAGCAGTCCATCTCAG
GTTTGAAATTGATATGGTGGCTCATTCTCTCTGTTACTTCGGAGGAGGTGATGCGGAAAA
GGCAGAGCTTGATGCTTACCGGGAAAAGCATTTCCCGACGTTGGCAAATCTAACAAAGAC
GCAAAAGTTTGTTCCTTTCCTCTCTTAACTCTTTTTCACATTATTATTTCAGTTTTGGTA
TCTCACTGAATGCCCTTTTGTTTTCTCTTATGCAGAATGCCATCTCCAGACGATCTGAGG
ACAGAAGGACTTTGTCCTTTATCACCTGAAGAAGCCGTGCTTATGCTTGCGGGTCTGGGT
TTTAGTCGGAAGACACGTGTTTTCGTCGCTGGTGCGAATATATATGGTGGGAATAAACGG
TTAGCAGCTTTAACGAGTCTCTACCCGAATCTAGTCACCAAAGAGAATGTACTCTCACAA
ACTGAGCTAGAGCCTTTCAAGAACTTCTCATCTCAGGTGAAATGAAAACAAGAAAACTTC
CGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTA
ATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCG
   
Sequence Name 23-K113530-0022-259-H02C-8409
GenBank Accession LN496635 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>23-K113530-0022-259-H02C-8409
TTCACCTGAGATGAGAAGTTCTTGAAAGGCTCTAGCTCAGTTTGTGAGAGTACATTCTCT
TTGGTGACTAGATTCGGGTAGAGACTCGTTAAAGCTGCTAACCGTTTATTCCCACCATAT
ATATTCGCACCAGCGACGAAAACACGTGTCTTCCGACTAAAACCCAGACCCGCAAGCATA
AGCACGGCTTCTTCAGGTGATAAAGGACAAAGTCCTTCTGTCCTCAGATCGTCTGGAGAT
GGCATTCTGCATAAGAGAAAACAAAAGGGCATTCAGTGAGATACCAAAACTGAAATAATA
ATGTGAAAAAGAGTTAAGAGAGGAAAGGAACAAACTTTTGCGTCTTTGTTAGATTTGCCA
ACGTCGGGAAATGCTTTTCCCGGTAAGCATCAAGCTCTGCCTTTTCCGCATCACCTCCTC
CGAAGTAACAGAGAGAATGAGCCACCATATCAATTTCAAACCTGAGATGGACTGCTAGAT
ACTTAGAAGCCTTATGGAGAGGACCTGCTTTCTTGTCCAATATGAATGACGCANATTTTG
GACCGACGAGATACGGGTCAACTGGTGCAAGGTGTGATCCACTATCTCGTAATCTCCTTA
CGAGTATCGCACCCGTTTCTTGTATCTTCGGAACGAAG

Last Updated on 06.12.2018 16:11