Sequences from confirmation amplicon


Confirmation sequences for line 260E01 and hit At5g36690 are shown.
Line ID 260E01
Locus At5g36690 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:14416623
Primers used for PCR RP07 - 8474, show primer details
   
Sequence Name 39-K024065-022-260-E01C-8409
GenBank Accession LN503217 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>39-K024065-022-260-E01C-8409
TAGATTTCCCGGACATGAAGCCATTTACAATTGTAGCCTGATGGGATCATTCTAAGTGAG
AAAGAAGCTTCCATAAATCGTGTCAAGGTAAACTCTAACTCTTTGTGTGATTTGCTAAAA
CATTGTTGAATCTAATGTTGTGATGGTTCTTTTGCCTCATCATTAGTCTATGGCTTTGGA
ATTGAATGAAGTAAAGAATGAGCTTGATGCGATTACTTGGAAGATAAACGACCTAAGTGA
CAAATTGTGGAAGAGTCAAAACAACATTAGGGTTTTGGATTTGGAAAAGGCACACATACT
TGAGAAGAGGGATAGATTTTATGCAAGGATTAAGATGTTGAGGATACAACGAGACAAAGG
AGTAAGATATTTGTCTTTCTGTTAATTTTTGCTTGTGGGTGATCTTAAAGATCTAATAAC
ATTTCGGTATATGCTTCTCGTGTTTGTGTAGAATGCTGCATTTTTCCAGAGCCTTTCTGT
TATGTGTAAAGCTAAAGAATTGGCTGCTTCTGGAAATGTTAGAGAGCTTGAAGTGTTTGC
TAGCTCTGAGGTTAGTTTGTTTGTTTGGTCTAAACGAAACATTGATAGAGAGAGATTTGA
GCATTTGTTCTTGGTTCTGTCTTTTTATAGGTTGATAGATTCATGACTCTTTG
   
Sequence Name 40-K024065-022-260-E01C-RP07
GenBank Accession LN503572 [GenBank]
Sequencing primer gene specific primer, RP07: 5'-AAGCCTTATCATCATTCCAAAGAG-3'
Sequence (A. th genome BLAST matches underlined) 
>40-K024065-022-260-E01C-RP07
CTATCAACCTATAAAAAGACAGAACCAAGAACAAATGCTCAAATCTCTCTCTATCAATGT
TTCGTTTAGACCAAACAAACAAACTAACCTCAGAGCTAGCAAACACTTCAAGCTCTCTAA
CATTTCCAGAAGCAGCCAATTCTTTAGCTTTACACATAACAGAAAGGCTCTGGAAAAATG
CAGCATTCTACACAAACACGAGAAGCATATACCGAAATGTTATTAGATCTTTAAGATCAC
CCACAAGCAAAAATTAACAGAAAGACAAATATCTTACTCCTTTGTCTCGTTGTATCCTCA
ACATCTTAATCCTTGCATAAAATCTATCCCTCTTCTCAAGTATGTGTGCCTTTTCCAAAT
CCAAAACCCTAATGTTGTTTTGACTCTTCCACAATTTGTCACTTAGGTCGTTTATCTTCC
AAGTAATCGCATCAAGCTCATTCTTTACTTCATTCAATTCCAAAGCCATAGACTAATGAT
GAGGCAAAAGAACCATCACAACATTAGATTCAACAATGTTTTAGCAAATCACACAAAGAG
TTAGAGTTTACCTTGACACGATTTATGGAAGCTTCTTTCTCACTTAGAATGATCCCATCA
GGCTACAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGA
TGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAG
ATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCC
GTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCT
Primers used for PCR RP08 - 8474, show primer details
   
Sequence Name 07-K024066-022-260-E01C-8409
GenBank Accession LN489087 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>07-K024066-022-260-E01C-8409
TAGATTTCTCCTTAAGCATCCAATCTTCTTCCTCCAAACCAATGCTACCATATTCAATCT
GATACTGAGCTATATAACTCTGCATCCACCACACCAAAACACTTCTACAGTCAAAAACCT
AACCGAATTTTAACGAATGGTTAGATAAAACAGTTGTATTACAAGATGATCAAGTTCCTC
CTTTGTAAAACAAAGTTGATCACTAGTAGTGCACCGGAGATTCCGTAGAGCTTCAAGTAG
AGTATCAAACTCCTTCTTCTTCTCTTCAATAACCATTCTATATCCTTCAGATTGAGGAAC
CAAACTCTCCATTTGAGTATACAAAGACGAAAACCTCAGCCAGTTCGGATTTCTTGGCGC
TCAAGAACATTGATTCGGAGTTCATTACAACGAAAAATC
   
Sequence Name 08-K024066-022-260-E01C-RP08
GenBank Accession LN489551 [GenBank]
Sequencing primer gene specific primer, RP08: 5'-GCTAATCAGGAGATTTTTCGTTGT-3'
Sequence (A. th genome BLAST matches underlined) 
>08-K024066-022-260-E01C-RP08
ATGAACTCCGAATCAATGTCTTGAGCGCCAAGAAATCCGAACTGGCTGAGGTTTCGTCTT
TGTATACTCAAATGGAGAGTTTGGTTCCTCAATCTGAAGGATATAGAATGGTTATTGAAG
AGAAGAAGAAGGAGTTTGATACTCTACTTGAAGCTCTACGGAATCTCCGGTGCACTACTA
GGGATCAACTTTGTTTTACAAAGGAGGAACTTGATCATCTGGTAATACAACTGTTTTATC
TAACCATTCGTTAAAATTCGGTTAGGTTTTTGACTGTAAAATTGTTTTGGGGTGGTGGAT
GCAAAG

Last Updated on 06.12.2018 16:11