Sequences from confirmation amplicon


Confirmation sequences for line 261A05 and hit At1g51810 are shown.
Line ID 261A05
Locus At1g51810 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:19227435
Primers used for PCR PP24 - 8474, show primer details
   
Sequence Name 27-K103231-0022-261-A05C-8409
GenBank Accession LN498344 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>27-K103231-0022-261-A05C-8409
TCGGAATGAGCCTTTACATTGAATATATCCGGACATATTGTATTATTGGTGATGATTACA
AACCAACCCGTGATAGACCAAAACCGCGAAAAGAGACATATAGCAGAATGGGTGGGAGGA
ATGCTTACGAAAGGAGACATCAAAAGTATTACTGATCCAAATCTCCTTGGAGATTATAAT
TCTGGTTCTGTTTGGAAAGCTGTTGAACTAGCAATGTCATGTATGAATCCTTCTTCGATG
ACAAGACCGACAATGTCTCAAGTTGTTTTTGAACTGAAAGAGTGTTTGGCCTCTGAAAGC
TCCAGGGAAGTGAGCATGACCTTCGGAACTGAAGTGGCCCCTATGGCTCGGTAGAGGTCC
TGATTATATTTCCTATTGTATCGCGTGGATCATTATTTTACCCTATATATCCTTTACAAC
TATCCTAAATTTTGTGCATCAATTTTATTTTTTGTAAGCAATGAACTTCTAATTACAATA
GAAGTTCAAAGTCTTTTTTCCCCCATGTTTCAGTTTATTCCAACTAACCATGAATTAAAT
AATGGGTGGTGGTGCATGTTTCTTTTACCAAAAATAACCATGAATTAATAACTATNGCAT
TTGCTTATGACCC
   
Sequence Name 75-K103232-0022-261-A05C-PP24
GenBank Accession LN513225 [GenBank]
Sequencing primer gene specific primer, PP24: 5'-AGAAGTTGAAGGGTCATAAGCAAA-3'
Sequence (A. th genome BLAST matches underlined) 
>75-K103232-0022-261-A05C-PP24
GGTATTTTTGGTAAAAGAACATGCACCACCACCCATTATTTAATTCATGGTTAGTTGGAA
TAAACTGAAACATGGGGGAAAAAAGACTTTGAACTTCTATTGTAATTAGAAGTTCATTGC
TTACAAAAAATAAAATTGATGCACAAAATTTAGGATAGTTGTAAAGGATATATAGGGTAA
AATAATGATCCACGCGATACAATAGGAAATATAATCAGGACCTCTACCGAGCCATAGGGG
CCACTTCAGTTCCGAAGGTCATGCTCACTTCCCTGGAGCTTTCAGAGGCCAAACACTCTT
TCAGTTCAAAAACAACTTGAGACATTGTCGGTCTTGTCATCGAAGAAGGATTCATACATG
ACATTGCTAGTTCAACAGCTTTCCAAACAGAACCAGAATTATAATCTCCAAGGAGATTTG
GATCAGTAATACTTTTGATGTCTCCTTTCGTAAGCATTCCTCCCACCCATTCTGCTATAT
GTCTCTTTTCGCGGTTTTGGTCTATCACGGGTTGGTTTGTAATCATCACCAATAATACAA
TATGTCCGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGC
AATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATT
CAAAAATGTAGATGTCCGCAGCGTTNTTATAAAATGAAAGTACATTTTGATAAAACGACA
AATTACGATCCGTCGTATTTATNGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAAT
CTTTATNTCGACGTGTCTA

Last Updated on 06.12.2018 16:11