Sequences from confirmation amplicon


Confirmation sequences for line 261B09 and hit At3g61900 are shown.
Line ID 261B09
Locus At3g61900 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:22926060
Primers used for PCR 30pv - 8474, show primer details
   
Sequence Name 28-K116770-0022-261-B09C-30pv
GenBank Accession LN499008 [GenBank]
Sequencing primer gene specific primer, 30pv: 5'-TTTGAAATCAACGGAGAATCTCTT-3'
Sequence (A. th genome BLAST matches underlined) 
>28-K116770-0022-261-B09C-30pv
TATAGGGGATTATTTGTTTTTGTCGTATAAGGGTATTTATTTATTAGTGACAGTTTTCAA
ATTTATACGTCTCTTAACAAAAATATCATATACGCAAATTGCCAAAACTTGTTCGTCATC
TCTCGCTACCATCAATCGGATAAACATATATCACAAGAAAAATTATACGAACAATTAATT
TATAATCTCACACTTATTTATATTTATGGATATTTCCAAACACCACTAGAACTTCTACTC
CTAATCTTGTGGGTTAGGCCGTGTTTGGCTCCACAACTCCACAAGATCAAACCCATTAGA
AAAGAAAGGAAAAACAAAACAAAAAGAAGAAACTAAATATGAAACGCAAATTTTTACACC
CCGTTTGCTTGCTTGCATCATCATCATCATCAAGCTCTAAAACATCCAACAAGATGATTA
TGACTTTGATTATGACGATGATCATGATGGTTGTTGTGGTAATTAACCGATCTCTCTCTG
TTGATCATGTCTTGAACATATCGAAAAACTTCCACGTGGCAAGTAAATAAAAAACATATC
CGACGACATAACATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAG
CAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAAT
TCAAAAATGTAGATGTC
   
Sequence Name 04-K116769-0022-261-B09C-8409
GenBank Accession LN488077 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>04-K116769-0022-261-B09C-8409
ATTTACATTGAATATGTTATGTCGTCGGATATGTTTTTTATTTACTTGCCACGTGGAAGT
TTTTCGATATGTTCAAGACATGATCAACAGAGAGAGATCGGTTAATTACCACAACAACCA
TCATGATCATCGTCATAATCAAAGTCATAATCATCTTGTTGGATGTTTTAGAGCTTGATG
ATGATGATGATGCAAGCAAGCAAACGGGGTGTAAAAATTTGCGTTTCATATTTAGTTTCT
TCTTTTTGTTTTGTTTTTCCTTTCTTTTCTAATGGGTTTGATCTTGTGGAGTTGTGGAGC
CAAACACGGCCTAACCCACAAGATTAGGAGTAGAAGTTCTAGTGGTGTTTGGAAATATCC
ATAAATATAAATAAGTGTGAGATTATAAATTAATTGTTCGTATAATTTTTCTTGTGATAT
ATGTTTATCCGATTGATGGTAGCGAGAGATGACGAACAAGTTTTGGCAATTTGCGTATAT
GATATTTTTGTTAAGAGACGTATAAATTTGAAAACTGTCACTAATAAATAAATACCCTTA
TTACGACAAAAACAAATTAATTCCCTTATTATATCTGTTAGGAGAAAAAAGAAGAAGAGA
TTCTCCGTTGAATTTCAAA

Last Updated on 06.12.2018 16:11